Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal con...

Astrocytomas are tumors which arise from astrocytes, cells that form the blood-brain barrier. There are very few drugs that successfully treat brain tumors. In this study, the cytotoxic effects on the HTB-12 astrocytoma cell line by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were studied. The presence of the TRAIL receptors, Death receptor 4 (DR4) and Death receptor 5 (DR5)...

AIM To analyse the effect of trypan blue on the retina in an experimental setting of macular surgery. METHODS Porcine eyes were used within 3 hours after death. The eyes were hemisected and the vitreous removed. Trypan blue (0.15%) was applied over the trephined posterior pole, whereas the rest of the eye cup was filled with a balanced salt solution (BSS). The dye and the BSS were removed aft...

OBJECTIVE To evaluate the value of microarthroscopy in the equine midcarpal joint using the vital stains methylene blue, trypan blue, neutral red, and Janus green B to observe components of the synovial lamina propria, vascular architecture, and synoviocytes. STUDY DESIGN Experimental. ANIMALS Ten horses. METHODS Microarthroscopy of left and right midcarpal joints was performed with and w...

Materials and methods: Th e eff ects of HBO on Leishmania tropica promastigote and amastigote forms were studied at a pressure of 2.5 atmosphere absolute (ata). We examined the cytotoxic eff ect of HBO on promastigotes using the trypan blue exclusion test and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye-reduction assay. To investigate the eff ects of HBO treatment ...

A method for simultaneously detecting membrane permeability (characteristic of necrosis) and DNA fragmentation (characteristic of apoptosis) is described. By combining a common dye-exclusion method (Trypan Blue) with a commercially available terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) labeling kit, we have succeeded in developing a novel methodology for o...

PURPOSE For intracytoplasmic sperm injection in the absence of sperm motility it can be virtually impossible to distinguish viable from nonviable sperm. A reliable means to identify viable nonmotile sperm is needed and would likely improve the intracytoplasmic sperm injection success rate. Optoelectronic tweezers are a new technology that uses light induced dielectrophoresis fields to distingui...

One approach to the study of cancer is the investigation of natural or acquired host defense mechanisms. In 1932 Foulds (6) compared the distribution of intravenous (I.V.) transplants of Brown-Pearce carcinoma in trypan blue-injected rabbits with that in controls. He found an increased incidence of splenic, hepatic, and pulmonary growths in trypan blue-treated animals and suggested that phagocy...

The effects of carrageenan and trypan blue on the expression of adoptive immunity to the syngeneic guinea pig line 10 hepatoma were investigated. Adoptive immunity was assessed by observing dermal tumor growth in recipients of immune cells and by bioassays in which tumor challenge sites were transplanted into secondary hosts. Carrageenan abrogated transferred immunity in treated animals as evid...

VARIOUS malformations in rats and mice have been observed to be caused by administration of the vital dye, Trypan blue (Gillman, Gilbert, Gillman, & Spence, 1948; Fox & Goss, 1956, 1957; Hamburgh, 1952, 1954; Waddington & Carter, 1952, 1953). Recently Waddington & Perry (1956) reported a teratogenic effect of Trypan blue on Amphibian embryos. The present paper deals with the effects of this dye...