PURPOSE To evaluate the effect of intravitreal azithromycin on the retina in a newborn rabbit model. METHODS Twelve, two-week old New Zealand albino rabbits were divided into two groups (six in each). The right eyes of six rabbits received 0.75 mg (0.05 mL) azithromycin and the right eyes of the remaining six rabbits 1.5 mg (0.1 mL) azithromycin intravitreally. Left eyes were served as the co...

Seizure causes neuronal cell loss in both animal models and human epilepsy. To determine the contribution of apoptotic mechanisms to seizure-induced neuronal cell death, rat brains were examined for the occurrence of terminal deoxynucleotidyl transferase-mediated UTP nick end labeling (TUNEL)-positive nuclei after pilocarpine-induced seizure. Numerous TUNEL-positive cells were observed througho...

The terminal deoxynucleotide transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) method for monitoring targeted cell ablation is based on the in situ labeling of DNA fragmentation sites in nuclei of intact fixed cells. Unlike other methods of detecting dying cells, the use of fixed material allows antigen expression to be monitored at the same time that apoptosis is confirmed in the targe...

Abstract The incidence rate of insomnia is increasing, but the mechanism it remains unclear. Warming Yang Strategy (WY) a kind Traditional Chinese Medicine, and proved to be effective in treating patients. animal was established with chlorophenylalanine (PCPA). Morris water maze open field test were performed evaluate influence WY on neurological recovery rats. TUNEL staining flow cytometry use...

This study aims to prove a high apoptotic index in the amniotic membrane as risk factor for preterm Labor. Case-control study, comparing and term groups (n=54) who underwent labor Obstetrics Emergency Room, Sanglah Central General Hospital Denpasar, Bali. Apoptotic is method of identifying assessing quantity tissue undergoing apoptosis, which this was tissue, calculated stained using TUNEL meth...

BACKGROUND/AIM It is known that trypan blue staining is not a good predictor of loss of corneal endothelial cells (ECs) during organ culture. As it is primarily an indicator of membrane integrity, it would also not be expected to identify ECs undergoing apoptosis. The aim of this study was to determine the ability of the in situ TdT dUTP mediated nick end labelling (TUNEL) technique to detect c...

Background/aim: It is known that trypan blue staining is not a good predictor of loss of corneal endothelial cells (ECs) during organ culture. As it is primarily an indicator of membrane integrity, it would also not be expected to identify ECs undergoing apoptosis. The aim of this study was to determine the ability of the in situ TdT dUTP mediated nick end labelling (TUNEL) technique to detect ...