The large-scale extraction and partial purification of endogenous 3',5'-cyclic UMP, 3',5'-cyclic IMP and 3',5'-cyclic dTMP are described. Rat liver, kidney, heart, spleen and lung tissues were subjected to a sequential purification procedure involving freeze-clamping, perchlorate extraction, alumina and Sephadex ion-exchange chromatography and preparative electrophoresis. The samples thus obtai...

Penelitian ini bertujuan untuk menganalisis pengaruh COVID-19 dan upah terhadap tingkat pengangguran terdidik di Indonesia dengan menggunakan analisis data panel yang terdiri dari cross section 33 provinsi time series kurun waktu 2010-2021. Hasil penelitian menunjukkan bahwa Upah Minimum Provinsi (UMP) riil tidak berpengaruh Indonesia. meningkatkan terbuka secara umum, terutama kurang terdidik....

BACKGROUND Encapsulated follicular tumours with equivocal papillary thyroid carcinoma (PTC) type nuclear features continue to remain a challenge despite the recent attempts to classify these borderline lesions. The term 'well differentiated tumour of uncertain malignant potential (WDT-UMP)' was introduced to classify these tumours. The present study aimed to evaluate the role of a cell cycle re...

A complementation test was devised to study allelism among the genetic determinants of resistance to 5-fluorocytosine in Candida albicans. Complementation was demonstrated in control hybrids produced by crossing a resistant strain that was deficient in cytosine deaminase activity with four other resistant strains deficient in UMP pyrophosphorylase activity. This complementation test was used to...

In this paper, I shall summarize a series of experiments that measure the effect of several inhibitors of uridine-5'-monophosphate (UMP) synthesis on the cellular level of activity for enzymes in the synthetic sequence. The inhibitors were administered to human diploid cell strains, growing in uitro, and to whole rats. In both instances, the change in specific activity of the cell or tissue pro...

We analyzed the expression of deoxycytidine kinase (dCK), UMP/CMP-kinase (UMP/CMP-K), nucleotide diphosphokinase (NDPK-B) and 5'-nucleotidases cN-II, cN-III, cdN and mdN by quantitative polymerase chain reaction at diagnosis in leukemic blasts from 96 patients with acute myeloid leukemia (AML) treated with ara-C. Our results show that high mRNA levels of cN-II and low mRNA levels of cN-III are ...

Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plate...

In Part II of this two-part paper, we analyze the marginal prices derived in Part I of this two-part paper within a robust optimization framework. The load and generation are priced at Locational Marginal Price (LMP) while the uncertainty and generation reserve are priced at Uncertainty Marginal Price (UMP). The Financial Transmission Right (FTR) underfunding is demonstrated when there is trans...

Macrocyclic ligands 1 and 2 containing two positively charged phenanthridinium units and aminobisacetylenic bridges, exhibit significantly higher affinity toward single-stranded rather than double-stranded polynucleotides. The ligands bis-intercalate into the former and show non-intercalative interactions with the latter type of nucleic acids. Both ligands differentiate AMP from the GMP or UMP ...