Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparat ions to reactive species which covalent ly bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicot inamide adenine dinucleot ide phosphate and is catalyzed by cytochrome P-450 monooxyge...

Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparat ions to reactive species which covalent ly bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicot inamide adenine dinucleot ide phosphate and is catalyzed by cytochrome P-450 monooxyge...

Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparat ions to reactive species which covalent ly bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicot inamide adenine dinucleot ide phosphate and is catalyzed by cytochrome P-450 monooxyge...

Hexamethylmelamine (HMM) is metabolized by rat hepatic microsomal preparat ions to reactive species which covalent ly bind to microsomal protein and to calf thymus DNA added to microsomal incubation mixtures. Covalent binding to macromolecules is dependent on the presence of molecular oxygen and reduced nicot inamide adenine dinucleot ide phosphate and is catalyzed by cytochrome P-450 monooxyge...

The persistence of Helicobacter pylori in the hostile environment of the human stomach is ensured by the activity of urease. The essentiality of Ni(2+) for this enzyme demands proper intracellular trafficking of this metal ion. The metallo-chaperone UreE promotes Ni(2+) insertion into the apo-enzyme in the last step of urease maturation while facilitating concomitant GTP hydrolysis. The present...

BACKGROUND The study and comparison of protein-protein interfaces is essential for the understanding of the mechanisms of interaction between proteins. While there are many methods for comparing protein structures and protein binding sites, so far no methods have been reported for comparing the geometry of non-covalent interactions occurring at protein-protein interfaces. METHODOLOGY/PRINCIPA...

Angiogenesis is the process of new blood vessel formation and is essential for a tumor to grow beyond a certain size. Tumors secrete the pro-angiogenic factor vascular endothelial growth factor, which acts upon local endothelial cells by binding to vascular endothelial growth factor receptors (VEGFRs). In this study, we describe the development and characterization of V21-DOS47, an immunoconjug...

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

The coadministration of phenol and hydroquinone has been shown to produce myelotoxicity in mice similar to that observed following benzene exposure. One explanation of this phenomenon may be that phenol enhances the peroxidase-dependent metabolic activation of hydroquinone in the mouse bone marrow. Here we report that radiolabeled [14C]hydroquinone and [14C]phenol bind covalently to tissue macr...