A stability-indicating method by micellar electrokinetic chromatography (MEKC) was developed and validated for determination of milnacipran (MNC) hydrochloride in capsules. The MEKC separation was performed in a fusedsilica capillary (40 cm x 50 μm i.d.), 30 kV voltage applied at 30 °C, using as background electrolyte solution 20 mM borate buffer (pH 8.8), 20 mM sodium dodecyl sulfate and detec...

A 21-residue peptide analog of the Ca2+ binding Site 3 in rabbit skeletal troponin C has been synthesized by the solid phase method. CD studies as well as uv difference spectroscopy did not show any changes upon addition of Ca2+ to this peptide in aqueous solution. Acetylation of the NH2-terminal residue of the analog resulted in Ca2+-induced CD and uv difference spectral changes and these chan...

In this article, we present our study on surface plasmon SP assisted contact scheme nanoscale photolithography technique. Sub-100-nm features on a metallic mask, fabricated by e-beam lithography, were successfully transferred to a resist pattern in a setup close to traditional photolithography. Our previous work based on finite difference time domain simulation reveals the mechanism of SP-light...

the present study describes a simple, accurate, precise and cost effective uv-vis spectroscopic and rp-hplc method for the estimation of ibandronate sodium (ibn). the determination of ibandronate sodium (ibn) was performed by both uv and rp-hplc method using 215 nm as the determination wavelength. the drug was dissolved in naoh solution (0.1n naoh) for estimation in uv and in distilled water fo...

Ultraviolet (UV) light generates two major DNA lesions: cyclobutane pyrimidine dimers (CPDs) and pyrimidine-(6-4)-pyrimidone photoproducts (6-4PPs), but the specific participation of these two lesions in the deleterious effects of UV is a longstanding question. In order to discriminate the precise role of unrepaired CPDs and 6-4PPs in UV-induced responses triggering cell death, human fibroblast...

This paper presents a method for evaluating the UV and biologically effective exposures to a plant canopy during the irradiation of soybean with supplemental levels of UV radiation in a greenhouse study. The method employs four materials as dosimeters that allow evaluation of the UV spectra. The exposures evaluated at three growth stages were less by factors of 0.44, 0.49 and 0.56 compared to t...

Apoptosis plays an important role in eliminating cells from populations when cells have been exposed to UV irradiation and damaged. Studies of cells in culture have provided some details of the mechanisms involved when stress response genes act after exposure to UV irradiation and other environmental stresses. However, little is known about the responses of intact sections of human skin growing...

Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) constitutes approximately 10% of Bacillus subtilis spore dry weight and has been shown to play a significant role in the survival of B. subtilis spores exposed to wet heat and to 254-nm UV radiation in the laboratory. However, to date, no work has addressed the importance of DPA in the survival of spores exposed to environmentally relevant...