The role of the REV3 gene function in UV-induced mutagensis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3--1 or rev3--3 allele. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations r...

Drake, John W. (University of Illinois, Urbana). Ultraviolet mutagenesis in bacteriophage T4. I. Irradiation of extracellular phage particles. J. Bacteriol. 91:1775-1780. 1966.-Ultraviolet (UV) irradiation of extracellular T4 phage particles induces about 2 x 10(-4)r mutations per lethal hit. The mutants largely escape detection unless the irradiated phages are plated with very soft overlay aga...

The global cellular response to UV-induced DNA damage has been analyzed in the p53-proficient human lymphoblastoid strain TK6 versus two isogenic derivatives wherein p53 activity was abrogated by diverse experimental approaches: (i) NH32, carrying a homozygous genetic knockout of p53; and (ii) TK6-5E, expressing the human papillomavirus E6 oncoprotein which binds and functionally inactivates p5...

Although using non-Saccharomyces yeasts during alcoholic fermentation can improve the wine aroma, most of them are not ethanol tolerant; therefore, in 2017, this study screened 85 isolated and identified from 24 vineyards seven Chinese wine-producing regions, obtaining Pichia terricola strain H5, which displayed 8% tolerance. Strain H5 was subjected to ultraviolet (UV) irradiation diethyl sulfa...

The yeast Saccharomyces cerevisiae appears to possess a single mutagenic or "error prony" pathway for the repair of UV damage; rev1, rev2, rev3 (Lemontt 1971a), rad6, rad8, rad9 and rad18 (Lawrence et al. 1974; present results). Strains carrying rad6 are the most sensitive to the lethal effects of UV light in this group and double mutants carrying rad6 and either rev1, rev3, rad9 or rad18 are n...