DNA gyrase is an essential bacterial enzyme required for the maintenance of chromosomal DNA topology. This enzyme is the target of several protein toxins encoded in toxin-antitoxin (TA) loci as well as of man-made antibiotics such as quinolones. The genome of Vibrio cholerae, the cause of cholera, contains three putative TA loci that exhibit modest similarity to the RK2 plasmid-borne parDE TA l...

The electronic structures and lattice dynamics of pressure-induced complex phase transitions [bcc --> hR1(110.5 degrees) --> distorted-hR1(108.2 degrees) --> bcc] in vanadium as a function of pressure up to 400 GPa have been investigated with an ab initio method using density functional perturbation theory (DFPT). At ambient pressure, the soft transverse acoustic phonon mode corresponding to Ko...

Nitrogen-doped tetrahedral amorphous carbon (ta-C :N) films were deposited by filtered cathodic vacuum arc (FCVA) technique using nitrogen as a background gas. The structure of the ta-C :N films was studied with X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy in terms of nitrogen flow rate used during the film deposition. Potential windows of the films measured in deaerated and un...

The cyclometallated tantalum(v) hydride complex {ArNC(Me)CHC(Me)N[2-(CHMeCH2)-6-(i)Pr-C6H3]}Ta(N(t)Bu)H (2) was prepared from hydrogenolysis of (BDI)TaN(t)BuMe2 (BDI = N,N'-diaryl-β-diketiminate, aryl = 2,6-(i)Pr2-C6H3). Based on mechanistic studies, formation of 2 likely proceeds through a dihydride intermediate generated from successive σ-bond metathesis steps. Compound 2 was found to undergo...

Tolfenamic acid Form I (CAS no.13710195, >98% purity) was purchased from Sigma Aldrich and used without further purification. TA Form II was crystallised by crash cooling an ethyl acetate solution (3.45 g TA Form I and 50.00 g of ethyl acetate) to 283.15 K. Both forms were isolated as pure phases as judged by their powder X-ray diffraction (PXRD) patterns. Ethyl acetate (EtOAc), ethanol and 2-p...

Transactional analysis (TA) is a theory of personality that can be applied in virtually all fields of psychology: educational, counseling, organizational and psychotherapy. The atmosphere that supports transactional analysis is one of comfort, security and respect. A positive relationship is forged between the clinician and the client in order to provide a model for subsequent relationships tha...