Clostridium perfringens iota-toxin consists of two separate proteins identified as a cell binding protein, iota b (Ib), which forms high-molecular-weight complexes on cells generating Na(+)/K(+)-permeable pores through which iota a (Ia), an ADP-ribosyltransferase, presumably enters the cytosol. Identity of the cell receptor and membrane domains involved in Ib binding, oligomer formation, and in...

Background and Aims: Adventitious agents, especially viral agents are among the most important concerns in viral vaccines. Viral contamination of biological products may arise from the original source of the reagents such as, serum, trypsin, animal or human derived media components or cell culture and working seed or cross contamination of vaccine during production. In this study, adventitious ...

In vitro cytotoxic effects of ZnO, FeO and Cu metallic nanopowders (NPs) on Vero (African green monkey kidney cell line), PK 15 (Pig kidney cell line) and Madin Darby Bovine Kidney (MDBK) cell lines were investigated at different time intervals (24 and 48 h). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effects of green synthesized...

The adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%)....

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a cont...

Background Vero cells are adherent cell lines commonly used for the production of viral vaccines. We had developed an animal component free medium that allows an optimal growth of this cell line in stirred bioreactor [1,2]. We had also showed that Vero cells grown in this medium (called IPT-AFM) sustained rabies virus replication, and resulted in an overall yield comparable to the level obtaine...

objective: reporter gene transfer to mammalian cells receives a great deal of attention due to its importance for molecular biology, embryology and developmental biology studies. among dna transfer technologies to eukaryotic cells, lipofection is known as the most widely used because of its easy handling procedure, low cell mortality and the natural pathway it undertakes. materials and methods:...

Cell culture requires a suitable medium and environment for in vivo conditions. Conditions are created by regulating temperature, pH, oxygen, CO2, osmotic pressure, surfaces to adhere cells, nutrients, vitamins, protection against toxic substances, hormones, growth factors that regulate cell differentiation. In general, good occurs at pH of 7.0 - 7.6. Fetal Bovine Serum (FBS) is one the supplem...

Background: Plants are a proven source of anti-tumor compounds and it is reasonable to assume that many such substances remain to be discovered. In Iranian folk medicine, ethanolic extract of the aerial parts of Daphne mucronata Royle is used against various skin disorders.Objective: In order to evaluate anti-neoplastic plants, we initiated a cytotoxic study using Daphne mucronata that is indig...

MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression post-transcriptionally. They play a critical role in developmental and physiological processes and have been implicated in the pathogenesis of several diseases including cancer. To identify miRNA signatures associated with different stages of neoplastic development, we examined the expression profile of 776 pri...