Dengue type 2 virus, strain New Guinea B, plaqued with equal facility and titer under overlays containing six different grades of commercial agar in the LLC-MK(2) cell system. Doubling the agar volume on LLC-MK(2) cell monolayers increased the plaque development time of dengue type 1, strain Hawaii. Storage of agar at 56 C reduced or totally abolished dengue type 4, strain H-241, plaque titer i...

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...

This report describes the preparation of a safe and immunogenic single vaccine against capripox disease in sheep and goats using two different types of cells (primary lamb kidney and vero, a bovine turbinate cell line). This used the 0240 pox strain isolated from sheep during an outbreak. The prepared vaccine was administered subcutaneously and induced complete protection against an experimenta...

Background Vero cells are adherent cell lines commonly used for the production of viral vaccines. We had developed an animal component free medium that allows an optimal growth of this cell line in stirred bioreactor [1,2]. We had also showed that Vero cells grown in this medium (called IPT-AFM) sustained rabies virus replication, and resulted in an overall yield comparable to the level obtaine...

this report describes the preparation of a safe and immunogenic single vaccine against capripox disease in sheep and goats using two different types of cells (primary lamb kidney and vero, a bovine turbinate cell line). this used the 0240 pox strain isolated from sheep during an outbreak. the prepared vaccine was administered subcutaneously and induced complete protection against an experimenta...

Introduction: Inactivated influenza vaccines are traditionally produced in chicken embryonated eggs but its limitations in producing the required doses in pandemic outbreaks quickly enough has made searching for alternative modes of production necessary. The use of cell culture-based vaccine production is one way of overcoming the limitations of the egg-based method and securing a more rapid re...

Coronavirus host and cell specificities are determined by specific interactions between the viral spike (S) protein and host cell receptor(s). Avian coronavirus infectious bronchitis (IBV) has been adapted to embryonated chicken eggs, primary chicken kidney (CK) cells, monkey kidney cell line Vero, and other human and animal cells. Here we report that acquisition of the cell-cell fusion activit...

Canine distemper virus (CDV) infections cause high morbidity and mortality in dogs. Changes the molecular biological characteristics of Korean CDV strain over multiple cell passages have not been reported. We investigated genetic CD1901-100 for use as an inactivated vaccine strain. Vero cells expressing dog nectin-4 gene (Vero/dNectin-4 cells) were used to adapt CD1901, which was passaged 100 t...

Using biological medium, such as plant extract for the biosynthesis of nanoparticles is an ecofriendly and emerging scientific trend. On this back drop the present study focused on the biosynthesis of silver nanoparticles using Cissus quadrangularis and evaluation of its antimicrobial and anticancer potentials. The biosynthesized nanoparticle was characterized by UV-VIS spectroscopy, EDAX and T...

New oxirane derivatives were synthesized using six naphthoquinones as the starting materials. Our biological results showed that these oxiranes acted as trypanocidal agents against Trypanosoma cruzi with minimal cytotoxicity in the VERO cell line compared to naphthoquinones. In particular, oxirane derivative 14 showed low cytotoxicity in a mammalian cell line and exhibited better activity again...