Recent measurements have indicated that some of the cardiac cell electrical capacitance is in series with a resistance. The computations of currents in a voltage clamp presented below show that, in this case, there is a danger that capacitive transient currents recorded during voltage clamp experiments may be confused with currents arising through rapid active membrane conductance changes. Seco...

The use of a discontinuous single electrode voltage-clamp (dSEVC) offers an attractive alternative to the patch-clamp technique, since whole-cell measurements can be performed with a single sharp electrode. Comparison of current-voltage relations, however, revealed a weaker voltage dependence of channels measured with the dSEVC compared to patch clamp. The accuracy of the dSEVC was tested on Vi...

Instantly switch recording modes by using the membrane potential as the trigger. Preset a voltage threshold in current-clamp mode, and MultiClamp 700B automatically switches to voltage-clamp when the membrane potential reaches that voltage level. User-specified delay allows you to perform creative new experiments. Suppress Oscillations Sudden changes in membrane or pipette parameters may result...

With the consideration of low standby leakage in nanoscale CMOS processes, a new 2×VDD-tolerant ESD clamp circuit was presented in this paper. The new ESD clamp circuit had a high-voltage-tolerant ESD detection circuit to improve the turn-on efficiency of the silicon-controlled-rectifier-based (SCRbased) ESD device. This design had been successfully verified in a 65-nm CMOS process. The leakage...

Voltage-clamp experiments of eight oligoester bolaamphiphiles in two subclasses are described. Syntheses of three new terephthalate-based compounds were achieved in three linear steps. Together with five previously described, related compounds, the ion transport activity was assessed by means of the voltage-clamp technique. All of the compounds show multiple types of conductance behavior in pla...

In an earlier experimental study, intracellular recording suggested that cholecystokinin (CCK) suppresses a K+ conductance in thalamic reticular (RE) neurons, yet the reversal potential of the CCK response, revealed using voltage clamp, was hyperpolarized significantly relative to the K+ equilibrium potential. Here, biophysical models of RE neurons were developed and used to test whether suppre...