The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates stu...

System administrators have utilized log analysis for decades to monitor and automate their environments. As compute environments grow, and the scope and volume of the logs increase, it becomes more difficult to get timely, useful data and appropriate triggers for enabling automation using traditional tools like Swatch. Cloud computing is intensifying this problem as the number of systems in dat...

We have successfully developed a Loop-mediated isothermal amplification (LAMP) assay that could specifically detect generic Escherichia coli (E. coli). This assay was tested on 85 bacterial strains and successfully identified 54 E. coli strains (average threshold time, Tt = 21.26). The sensitivity of this assay was evaluated on serial dilutions of bacterial cultures and spiked faeces. The assay...

A study was designed to investigate management factors that might influence the shedding of verocytotoxin-producing Escherichia coli (VTEC) O157 by beef cows in Scotland, where there is a particularly high rate of human infection. Thirty-two herds were visited at least monthly over approximately 1 year for collection of fresh faecal pat samples and information on management factors. The faecal ...

The large outbreak occurred in Germany during Summer 2011 associated to the consumption of sprouts was caused by a VTEC strain which possessed atypical characteristics, and did not fit the current definition of pathogenic VTEC. In fact, the epidemic strain did not possess the eae gene, encoding the adherence factor intimin, but possessed genes that are typical of the Enteroaggregative Escherich...

In a significant proportion of cases of community acquired diarrhoea no recognised pathogen is detected. Verocytotoxin producing Escherichia coli (VTEC) are associated with haemorrhagic colitis and the haemolytic uraemic syndrome but their role in community acquired diarrhoea is not fully understood. Using a method of toxin enhancement in mixed faecal culture, 175 stools negative on culture for...

[1] In order to make best use of the opportunities provided by space missions such as the Radiation Belt Storm Probes, we determine the response of complementary subionospheric radiowave propagation measurements (VLF), riometer absorption measurements, cosmic noise absorption, and GPS-produced total electron content (vTEC) to different energetic electron precipitation (EEP). We model the relati...

To the Editor—We read with interest the recent paper by Jelacic et al. [1], in which they unsuccessfully sought a link between ABO and P1 blood groups and outcomes of childhood infections by Escherichia coli O157. We would like to draw readers’ attention to our observations during an outbreak of hemolytic-uremic syndrome (HUS), in which we compared the numbers of seroreactivities to potential e...