A machine vision based approach for classification of tobacco leaves for automatic harvesting in a complex agricultural environment is proposed in this paper. The CIELAB color space model is used to segment the leaf from the background. The segmented leaves are classified into three classes viz., ripe, unripe, and over-ripe. Models based on various textural features such as GLTP (Gray Level Loc...

The preparation, certification, and characterization of two new biological certified reference materials for inorganic trace analysis have been presented. They are based on two different varieties of tobacco leaves, namely, Oriental Basma Tobacco Leaves (INCT-OBTL-5), grown in Greece, and Polish Virginia Tobacco Leaves (INCT-PVTL-6), grown in Poland. Certification of the materials was based on ...

The definition of "minor" veins in leaves is arbitrary and of uncertain biological significance. Generally, the term refers to the smallest vein classes in the leaf, believed to function in phloem loading. We found that a galactinol synthase promoter, cloned from melon (Cucumis melo), directs expression of the gusA gene to the smallest veins of mature Arabidopsis and cultivated tobacco (Nicotia...

In a recent study of N-acylphosphatidylethanolamine (NAPE) metabolism in elicitor-treated tobacco (Nicotiana tabacum L.) cells, we identified a rapid release and accumulation of medium-chain N-acylethanolamines (NAEs) (e.g. N-myristoylethanolamine or NAE 14:0) and a compensatory decrease in cellular NAPE (K.D. Chapman, S. Tripathy, B. Venables, A.D. Desouza [1998] Plant Physiol 116: 1163-1168)....

Abstract Background Weather change in high-altitude areas subjects mature tobacco ( Nicotiana tabacum L.) to cold stress, which damages leaf yield and quality. A brupt diurnal temperature differences (the daily dropping more than 20 °C) along with rainfall tobacco-growing at an altitude above 2450 m, caused stress field-grown tobacco. Results After the flue-cured suffered field, surface color o...

Cysteine proteinases are involved in various physiological and developmental processes in plants. Two cDNAs from senescent and non-senescent tobacco leaves were isolated with degenerate primers designed from conserved regions of plant senescence-associated cysteine proteinases using rapid amplification of cDNA ends (RACE). Both sequences encode papain-like cysteine proteinases: the 833 bp fragm...

Tobacco products, including cigarettes, cigars, chewing tobacco, snuff, and loose pipe tobacco, contain the dried, processed leaves of the tobacco plant Nicotiana rustica or Nicotiana tabacum. All forms of tobacco contain nicotine, an extremely addictive drug that can act as both a central nervous system stimulant and depressant. In addition to nicotine, tobacco contains thousands of other chem...