Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

This paper explores how the dongba as the ritual practitioner perceives his authenticity during the marriage ceremony in the Naxi Wedding Courtyard in Lijiang, China. Through the dongba’s life story, the paper indicates that, the power of his making judgment is not entirely related to the toured objects, socially constructed reality or the existential feeling, but also has to do with what happe...

Background. Complications of spinal anaesthesia (SpA) range between 1 and 17%. Habitus and operator experience may play a pivotal role, but only sparse data is available to substantiate this claim. Methods. 161 patients were prospectively enrolled. Data such as spread of block, duration of puncture, number of trials, any complication, operator experience, haemodynamic parameters, was recorded a...

Abstract Remarkable efforts have been made in Translation and Interpreting Studies to test the subservient habitus hypothesis formulated by Simeoni (1998) his seminal work. In face of increasing evidence that translators tend reproduce a given society’s or community’s prevalent norms contribute stability such ( Toury 1978 ), subversive translation practices reported Delabastita 2011 ; Woods 201...

Building on Marx and Weber, Bourdieu developed a sociology for scrutinizing the processes of domination accumulation that allow social reproduction to take place. Yet, rarely tackled breakdowns orders never construed war as scientific object, even if he signaled theoretical interest in an inverse sociogenesis state. Despite this limitation, we argue his work furnishes conceptual instruments thi...