We re-tested stored (frozen) DNA samples in 5 independent polymerase chain reaction (PCR) replicates and confirmed that equivocal test results from a previous study on white spot syndrome virus (WSSV) in brooders and their offspring arose because amounts of WSSV DNA in the test samples were near the sensitivity limits of the detection method. Since spawning stress may trigger WSSV replication, ...

White spot syndrome virus (WSSV), is the most contagious pathogen of cultured shrimp that causes mass mortality, leading to huge economic loss to the shrimp industry. The lack of effective therapeutic or prophylactic measures has aggravated the situation, necessitating the development of antiviral drugs. With this objective, the antiviral activity of the drug, (TP22C derived from the terrestria...

Taking an innovative approach, a vaccination study using five bacterial strains viz. Vibrio campbelli (B60), V. alginolyticus (B73), V. parahaemolyticus-like (B79), V. parahaemolyticus (R8) and V. harveyi (RG203) was conducted in Penaeus monodon against white spot syndrome virus (WSSV) infection, considered as one of the serious pathogens of shrimps. Oral challenge with shrimps infected with WS...

One of the lessons ultimately learned from the first dramatic outbreaks of white spot syndrome virus (and other shrimp viral diseases) in the early 1990’s was that aquaculture management practices needed to be improved. Subsequent research revealed that critical factors included: broodstock sourcing, postlarva sourcing, screening techniques and strategies, diverse transmission pathways, critica...

Among the important challenges to shrimp aquaculture worldwide are the diseases caused by viruses, in particular by white spot syndrome virus (WSSV), which has a genome estimated to contain 305 kb. By analysis and comparison of the WSSV genomic DNA and cDNA libraries, an ORF (vp28 gene) was identified. The gene, encoding a novel 204-amino-acid protein, was expressed in Escherichia coli and puri...

A protein of 110 kDa (termed VP110) from the envelope fraction of White spot syndrome virus (WSSV) was identified by SDS-PAGE and mass spectrometry. The resulting amino acid sequence matched an open reading frame (wsv035) containing an Arg-Gly-Asp (RGD) motif in the WSSV genome database. To validate the mass-spectrometry result, the C-terminal segment of the wsv035 open reading frame was expres...

BACKGROUND RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA." A co-culti...

For more than three decades, infectious diseases have been one of the major causes economic losses in global shrimp industry. One deadliest pathogens is white spot syndrome virus (WSSV), which mass mortality farmed shrimp. Various aspects WSSV pathogenicity studied, but this review, we will focus only on them, namely metabolic reprogramming. WSSV’s modulation host metabolism affects glycolysis,...

Although aquaculture in Ecuador has a high economic and socio-cultural importance, pathogenic microorganisms affect the development vitality of crustaceans, fish, mollusks, reducing their production yields. Among these pathogens, White Spot Syndrome Virus (WSSV) is an invertebrate virus that induces mortality, generating severe losses due to its wide geographical distribution infection rate fin...

Viruses, in particular DNA viruses, generate microRNAs (miRNAs) to control the expression of host and viral genes. Due to their essential roles in virus-host interactions, viral miRNAs have attracted extensive investigations in recent years. To date, however, most studies on viral miRNAs have been conducted in cell lines. In this study, the viral miRNAs from white spot syndrome virus (WSSV) wer...