the present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza a m2-protein (m2e), which was fused to the n-terminus of the truncated hsp70 (hsp70359–610) molecule as a new approach for future vaccine research against influenza a. the amplified fragments, m2e and hsp70359-610 genes, were gel-purified. the products were then single digested with bam...

New cases of blue cheese discoloration has led to recent research identify the causal agent and factors that favor pigment appearing. Nonetheless, very few reports have described source contamination measurements eradicate microbiological on farms by determining relation between fresh Pseudomonas fluorescens group. Thus, 60 samples from a farm (cheese, equipment surfaces, tap water, raw pasteur...

background mycobacterium tuberculosis genotyping can effectively improve tuberculosis (tb) control programs by controlling disease transmission. pulsed field gel electrophoresis (pfge) is a particularly powerful tool for determination of clonal identity of bacteria providing information for understanding and controlling the spread of disease. objectives the aim of present study was to investiga...

Estrogen is a steroidal hormone involved in several physiological functions in the female body including regulation of serum lipid metabolism and breast cancer (BC). Estrogen actions on serum lipids mostly occur through its binding to intracellular Estrogen Receptor alpha (ERalpha) isoform, expressed in most of tissues. This gene (ESR1) exhibit many polymorphic sites (SNPs) located either on tr...

We report the emergence in Kenya during 1997-1999 of typhoid fever due to Salmonella enterica serovar Typhi resistant to ampicillin, tetracycline, chloramphenicol, streptomycin, and cotrimoxazole. Genotyping by pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA yielded a single cluster. The multidrug-resistant S. Typhi were related to earlier drug- susceptible isolates but were u...

A circular map of the 208-kilobase IncHII plasmid pHH1508a was constructed by using the restriction endonucleases XbaI, XhoI, and NotI. Tn5 insertion mutagenesis showed that transfer genes were distributed widely over the plasmid map. Analysis of the deletion mutant pDT1178 indicated that genes required for conjugative pilus production are located in a 96.0-kilobase region of the plasmid.

100 μL 2% (wt/vol) 5-bromo-4-chloro3-indolyl-β-D-galactopyranoside (Xgal) and 20 μL 100 mM isopropyl β-Dthiogalactopyranoside (IPTG). Individual blue colonies were selected and grown overnight in Superbroth containing 50 μg/μL ampicillin. Plasmid DNA was isolated using the PERFECTprep Kit (5 Prime→3 Prime, Boulder, CO, USA), and the construct was checked by restriction digestion and sequencing...