This review fundamentally covers the functional analysis of proteins and the preparation of modified proteins for the creation of novel functions using a yeast molecular display system as a molecular tool. Yeast molecular display is a genetic technique of producing proteins or peptides on the yeast cell surface, such as the cell wall or cell membrane. In this review, recent studies on the yeast...

The actin-associated protein Sla1p, through its SHD1 domain, acts as an adaptor for the NPFX(1,2)D endocytic targeting signal in yeast. Here we report that Wsc1p, a cell wall stress sensor, depends on this signal-adaptor pair for endocytosis. Mutation of NPFDD in Wsc1p or expression of Sla1p lacking SHD1 blocked Wsc1p internalization. By live cell imaging, endocytically defective Wsc1p was not ...

The aim of this study was to evaluate the usefulness of Saccharomyces cerevisiae cell wall preparations in the adsorption of ochratoxin A (OTA). The study involved the use of a brewer's yeast cell wall devoid of protein substances, glucans obtained by water and alkaline extraction, a glucan commercially available as a dietary supplement for animals and, additionally, dried brewer's yeast for co...

The pathogenicity of the opportunistic human fungal pathogen Candida albicans depends on its ability to escape destruction by the host immune system. Using mutant strains that are defective in cell surface glycosylation, cell wall protein synthesis, and yeast-hypha morphogenesis, we have investigated three important aspects of C. albicans innate immune interactions: phagocytosis by primary macr...

Background: Biosorption plays important roles in the recovery of rare earth metals. The absorption dysprosium (Dy) was tested yeast. Interestingly, brewing yeast, Saccharomyces cerevisiae, showed Dy absorption, and two strains, Alt-OF2 Alt-OF5—previously isolated as highly aluminum-tolerant -absorbing yeast strains—were screened shown to be superior terms their when compared S. cerevisiae. Here...