The present study explores the biochemical and pharmacological examination of H. agelenoides spider venom gland extract for the proteolytic activity and along with other pharmacological properties. The venom gland extract hydrolyzed casein, the proteolytic activity was further confirmed by the activity staining on casein zymogram. The venom gland extract showed two translucent activity bands on...

Abstract This work aimed to obtain aspartic proteases of industrial and biotechnological interest from the stomach crevalle jack fish (Caranx hippos). In order do so, a crude extract (CE) was obtained subjected partial purification by salting-out, which resulted in enzyme (EE) obtainment. EE were characterized physicochemically means zymogram. addition, effect chemical agents on their activity ...

Gingipains (RgpA, RgpB, and Kgp) are major virulence factors of the periodontitis-causing bacterium Porphyromonas gingivalis. Isolation gingipains from crude protein sample P. gingivalis is critical for studying underlying invasion mechanism that contributes to periodontitis, Alzheimer’s disease, cardiovascular disease (CVD). Chromatographic processes molecular cloning two standard techniques o...

Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDSfibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acryl...

background one group of significant enzymes produced by bacillus genus are alkaline proteases with several important applications in the daily life as well as common industries such as food, detergents, leather, alcohol and beer production and medical and sanitary industries, beside wastewater treatment, , biotransformation, hydrolyzed proteins and oil manufacturing. objectives in this paper, k...

Introduction Although isozyme markers may not be abundant in a species, their codominant inheritance has made them useful in certain situations. In linkage mapping experiments, codominant markers are often preferred over dominant markers as they carry more information on the recombination fraction than dominant markers in the F2 segregating population . An inheritance study will provide allelic...

Bacterial biocatalysts play a key role in our transition to a bio-based, post-petroleum economy. However, the discovery of new biocatalysts is currently limited by our ability to analyze genomic information and our capacity of functionally screening for desired activities. Here, we present a simple workflow that combines functional metaproteomics and metagenomics, which facilitates the unmediat...