We describe a gentle and rapid method to purify the intact multiprotein DNA replication complex using free flow electrophoresis (FFE). In particular, we applied FFE to purify the human cell DNA synthesome, which is a multiprotein complex that is fully competent to carry-out all phases of the DNA replication process in vitro using a plasmid containing the simian virus 40 (SV40) origin of DNA rep...

Optimized and validated spectrophotometric methods have been proposed for the determination of iron and cobalt individually and simultaneously. 2-hydroxy-1-naphthaldehyde-p-hydroxybenzoichydrazone (HNAHBH) reacts with iron(II) and cobalt(II) to form reddish-brown and yellow-coloured [Fe(II)-HNAHBH] and [Co(II)-HNAHBH] complexes, respectively. The maximum absorbance of these complexes was found ...

This paper describes the raising of the low front vowels /ɛ, æ/ and the lowering of the mid front /e/ before the voiced velar /ɡ/ in Pacific Northwest English (PNWE). This prevelar system appears to have two parts with differing social patterns. In the most advanced part, /ɛɡ/ and /eɡ/ are homophonous upgliding diphthongs merged at a point between non-prevelar /ɛ/ and /e/ for all 20 Caucasian S...

We consider a fundamental online scheduling problem in which jobs with processing times and deadlines arrive over time at their release dates. The task is to determine feasible preemptive schedule on single or multiple possibly unrelated machines that maximizes the number of complete before deadline. Due strong impossibility results for competitive analysis machine, we require contain some slac...

Nonthermal Brownian motion is investigated experimentally by injecting a particle into soft-mode turbulence (SMT), in the electroconvection of a nematic liquid crystal. It is clarified that the particle motion can be classified into two phases: fast motion, where particles move with the local convective flow, and slow motion, where they are carried by global slow pattern dynamics. We propose a ...

Measuring the viscoelastic behavior of highly hydrated biological materials is challenging because of their intrinsic softness and labile nature. In these materials, it is difficult to avoid prestress and therefore to establish precise initial stress and strain conditions for lumped parameter estimation using creep or stress-relaxation (SR) tests. We describe a method ( ɛ˙M or epsilon dot metho...

The randomly diluted resistor network and XY model at low temperature T are studied near the d-dimensional percolation threshold using the ɛ expansion, where ɛ=6-d. The series expansion of the inverse susceptibility in powers of T for the XY model is identical to that of the appropriate resistive inverse susceptibility in powers of σ0−1, where σ0 is the conductance of a bond. However, the tempe...

Motivated b y reliability considerations i n data deduplication for s torage s ystems, w e introduce the problem of flexible coloring. Given a hypergraph H and the number of allowable colors k, a exible coloring o f H is a n assignment o f o ne or more colors to each vertex s uch that, for each hyperedge, i t is possible to choose a color from each vertex's color list so that this hyperedge i s...

He and Rao (2013) reported a raising phenomenon of /a/ in /Xan/ (X being a consonant or a vowel) in Chengdu dialect of Mandarin, i.e. /a/ is realized as [ɛ] for young speakers but [æ] for older speakers, but they offered no acoustic analysis. We designed an acoustic study that examined the realization of /Xan/ in speakers of different age (old vs. young) and gender (male vs. female) groups, whe...