The β-glucosidase gene bgl3a from Myceliophthora thermophila, member of the fungal glycosyl hydrolase (GH) family 3, was cloned and expressed in Pichia pastoris. The mature β-glucosidase gene, which results after the excision of one intron and the secreting signal peptide, was placed under the control of the strong alcohol oxidase promoter (AOX1) in the plasmid pPICZαC. The recombinant enzyme (...

Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its production requires induction. A mixture glucose and β-disaccharide has been demonstrated to achieve high-level production. However, as inducers, β-disaccharides are prone be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore need supplemented through feeding ov...

Lower termites need symbiotic microbes for cellulose digestion. Elizabethkingia miricola strain BM10 has been proposed as a microbe that assists in low-temperature digestion and metabolism of Reticulitermes speratus KMT1, termite on Bukhan Mountain, Seoul, Korea. In E. BM10, β-glucosidase genes expressed at 10°C were identified, the psychrophilic enzymatic characteristic was confirmed by hetero...

Efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. The production of β-glucosidase, β-xylosidase and xylanase by Colletotrichum graminicola was optimized using Response Surface Methodology (RSM). Maximal production occurred in wheat bran. Sugarcane trash, peanut hulls and corncob enhanced β-glucosidase, β-xylosidase and...

Background: Cellulose is the most abundant biomass on earth. The major players in cellulose degradation in nature are cellulases produced by microorganisms. Aerobic filamentous fungi are the main sources of commercial cellulase. Trichoderma reesei has been explored extensively for cellulase production; however, its major limitations are its low β-glucosidase activity and inefficiency in biomass...

Inhibitors of carbohydrate-hydrolysing enzymes play an important role for the treatment of diabetes. One of the therapeutic methods for decreasing of postprandial hyperglycemia is to retard absorption of glucose by the inhibition of carbohydrate- hydrolysing enzymes, such as α-glucosidase, in the digestive organs. To investigate the therapeutic potential of compounds from natural sources, Calli...

Few microbial functions have been compared to a comprehensive survey of the human fecal microbiome. We evaluated determinants of fecal microbial β-glucuronidase and β-glucosidase activities, focusing especially on associations with microbial alpha and beta diversity and taxonomy. We enrolled 51 healthy volunteers (26 female, mean age 39) who provided questionnaire data and multiple aliquots of ...

The considerably low β-glucosidase activity : FPA ratio of 0.5-0.6 represented by the enzyme complex of Trichoderma reesei RUT C30 results in poor hydrolysis potential. To overcome this problem culture conditions were developed to enhance β-glucosidase production. Mandels’ medium supplemented with tris-maleate buffer proved to be efficient for cellulase production with elevated β-glucosidase ac...