Zinc oxide nanoparticles are highly sensitive towards phosphate ions even at pH 7. Buffer solutions and cell culture media containing phosphate ions are able to destroy ZnO nanoparticles within a time span from less than one hour to one day. The driving force of the reaction is the formation of zinc phosphate of very low solubility. The morphology of the zinc oxide particles has only a minor in...

OBJECTIVE To identify metabolites showing changes in serum levels among Japanese male with diabetes. METHODS We performed metabolite profiling by coupling capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry using fasting serum samples from Japanese male subjects with diabetes (n=17), impaired glucose tolerance (IGT; n=5) and normal glucose tolerance (NGT; n...

A particulate preparation isolated from Escherichia coli B catalyzes the acylation of 1-acyl-an-glycerol j-phosphate (I-acyl-GP) with both oleoyl-CoA and pahnitoyl-CoA. The optimum conditions were determined for the acyl-CoA: I-acyl-GP acyltransferase. The acyltransferase is specific for the I-acyl-GP and does not acylate 2-acyl-sn-glycerol 3-phosphate (2-acyl-GP) under the conditions used. The...

Previous clinical study showed that aged garlic extract (AGE) has a preventive effect of vascular calcification in patients with coronary artery diseases. It has been reported that vascular smooth muscle cells (VSMC) and inflammatory cells including macrophages and lymphocytes migrate to sub-intimal region where atherosclerotic plaques are formed, and VSMC can differentiate into osteoblast-like...

Glycosomes, purified from trypomastigote forms of Trypanosoma brucei, contained all the enzymes necessary to convert glucose to alpha-glycerophosphate and 3-phosphoglycerate. The multienzyme reaction which produces 2 alpha-glycerophosphate, 2 ADP, and 2 NAD+ from 1 glucose, 2 ATP, and 2 NADH was studied spectrophotometrically. Intact glycosomes, suspended with 5.6 mM alpha-glycerophosphate and ...

Phospholipids from Mycobacteria have an unusual fatty acid pattern; palmitic acid is mainly on position 2 while oleic acid is found predominantly at position 1 of glycerophospholipids (Okuyama, H., Kankura, T., and Nojima, S. (1967) J. Biochem. (Tokyo) 61, 732-737). Membrane preparations were obtained from Mycobacterium butyricum, which had acyl-CoA:glycerophosphate and acyl-CoA:monoacylglycero...

Glycerophosphate (GP)-dependent, ferricyanide-induced hydrogen peroxide production was studied in brown adipose tissue mitochondria from newborn rats. Relations between the rate of hydrogen peroxide production and total amount of hydrogen peroxide produced at different GP and ferricyanide concentrations were determined. It was found that the rate of hydrogen peroxide production increases with i...