OBJECTIVES: The purpose of this study was to determine the effects of estradiol and progesterone on interferon-γ (IFN-γ), interleukin (IL)-12, IL-10 and tumor necrosis factor-α (TNF-α) productions in polyclonal activators (phyto hemagglutinin+lipopolysaccharide)-stimulated whole blood cultures. METHODS : Nineteen healthy males and females volunteered in the study. Blood samples were drawn, dilu...

Objective: The interleukin-12/interferon-γ (IL-12/IFN-γ) pathway is the most validated cytokine regulating Mycobacterium tuberculosis infection. role of IL-12/IFN-γ axis in protecting against (TB) exhibited people having mutations genes encoding these cytokines. We aimed to study serum levels IL-12 and IFN-γ pediatric their correlation with clinical microbiological features. Material Methods: A...

Abstract Background The mechanisms of interindividual susceptibility to respiratory syncytial virus (RSV) are not clearly understood. Viral load (VL), an exaggerated immune response or T-helper 2 (Th2) bias were postulated as playing a role in the severity RSV infection. We aimed analyze VL, cytokine profiles and their association with life-threatening disease (LTD). Methods Prospective cohort ...

Objective(s):With regard to pharmacological effects of carvacrol on the respiratory system, its effect on cytokines genes expression in splenocytes of asthmatic mice was examined in this study. Materials and Methods:Splenocytes were isolated from non-sensitized (control group), sensitized mice to ovalbumin (OVA) (group S), and S animals treated with dexamethasone, and three concentrations of c...

Background: Several biological and medical benefits of Saffron, Crocus sativus (Iridaceae), have been demonstrated. However, mechanisms of actions for purified constituents are greatly unknown. Objective: To examine the effects of Safranal, a main constituent of Saffron stigma, on cell viability and cytokine profile of peripheral blood mononuclear cells (PBMC) were examined. Methods: Effect...

Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...

Interferon-γ (IFN-γ) production by natural killer (NK) cells and cytotoxic lymphocytes is a key component of innate and adaptive immune responses. Because inhibitor of κB-ζ (IκBζ), a Toll-like receptor (TLR)/interleukin-1 receptor (IL-1R) inducible transcription factor, regulates IFN-γ production in KG-1 cells, we tested IκBζ's role in the classic lymphocyte pathway of IL-12/IL-18-induced IFN-γ...

Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...

Background: IL-4 is a cytokine that induces differentiation of naive helper T cells into Th2 cells. Once activated by IL-4, Th2 cells subsequently produce additional IL-4. Objective: To examine the effect of IL-4 on IL-17 production and its effect in Collagen- Induced Arthritis (CIA) mice. Method: In this study, a chicken collagen-II-induced experimental arthritis (CIA) model was used in DBA/1 ...