Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...

Campylobacter jejuni was monitored in 4 chicken farms during the period 2003 to 2006 to elucidate the mechanisms of transmission. Three farms (1 to 3), located at least 14 km from each other, belonged to an integrated poultry company, which also provided the farms with day-old chicks from several hatcheries as well as chicken feed. Another farm (4), which belonged to a different company, was lo...

BACKGROUND We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to identify blood meals ingested by field female sand fly in the northwest of Iran. In addition, the utility of PCR-RFLP was compared with ELISA as a standard method. METHODS This experimental study was performed in the Insect Molecular Biology Laboratory of School of Public Health, Tehra...

BACKGROUND Efficient monitoring of endemic and resurgent visceral leishmaniasis (VL) requires discriminatory molecular tools that allow direct characterization of etiological agents (i.e., the Leishmania donovani complex) in host tissues. This characterization is possible through restriction fragment-length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified sequences (PCR...

A new monitoring system for bacterial communities involving dimethylarsinic acid (DMAA) decomposition was provided by combining the MPN (Most Probable Number) method and RFLP (restriction-fragment-length polymorphism analysis). The abundance of DMAA decomposing bacteria was estimated by the MPN method using a bacterial culture medium, which included DMAA as the sole carbon source, indicating ba...

Abstract. The purpose of the research Was to study genetic diversity bovine leukemia virus isolates obtained in Belgorod Region using restriction fragment length polymorphism method (RFLP). Scientific novelty. Bovine leukosis is one most common chronic infectious diseases cattle many countries world, which causes significant economic losses livestock industry. typing (BLV), its structure, evalu...

15 The genetic diversity of Frankia strains nodulating Alnus glutinosa along the basin of the Tormes River was 16 studied on DNA extracted directly from nodules. Frankia strains inhabiting root nodules at 12 different 17 locations, ranging in altitude from 409 to 1181 m, were characterized. For that, we amplified the whole IGS 18 region between 16S–23S rDNA and performed a restriction fragment ...

The recently described genus Pandoraea consists of five named species and four unnamed genomospecies, several of which have been identified in clinical specimens including respiratory secretions from persons with cystic fibrosis. We investigated whether it is possible to distinguish species of the genus Pandoraea by means of restriction fragment length polymorphism (RFLP) analysis and direct se...

Ž . We investigated the distribution of EcoRI restriction fragment length polymorphism RFLP and tested EcoRI and HindIII RFLPs to ascertain the occurrence of scrapie in Suffolk sheep. Genomic DNA samples were collected from 527 Ž . animals and typed for EcoRI RFLP by Southern blot analysis using the PrP gene clone probe pNPU42 . The average e1 frequency in Suffolk sheep was 64.4% and ranged fro...

BACKGROUND In 2009, 3 genome-wide association studies implicated IL28B single-nucleotide polymorphisms (SNPs) as the strongest genetic pretreatment predictor of sustained virological response (SVR) in hepatitis C infection. Recently, the American Association for the Study of Liver Diseases (AASLD) and the European Association for the Study of the Liver (EASL) included IL28B testing in their gui...