To determine whether hemoglobin regulation is normal in diseases affecting 1-globin gene expression. globin synthesis was examined in members of a family of a patient with hereditary persistence of fetal hemoglobin/fi#{176}-thalassemia (HPFH/$#{176}-thal). The HPFH defect is the Ghanian type II. with a deletion from t’$ to at least 20 kb 3’ to . The / #{176}-thaI gene has the haplotype II restr...

I N 1930, Brewster and Cannon’#{176} reported the first example of acute leukemia in a case of mongolism ( Down’s syndrome ) . Between 1947 and 1963, further reports appeared demonstrating the increased incidence of acute leukemia in Down’s syndrome.7”’#{176}”5’48’49’6#{176}’65’M7’#{176}’ In 1958, Stewart et al.TM5 reported that of 677 children dying of leukemia, 18 were associated with Down’s ...

In this fast, simple method for analysis of microgram amounts of purine and pyrimidine bases in nucleic acid hydrolysates, RNA and DNA are optimally hydrolyzed in equal volumes of trifluoroacetic acid and formic acid, heated in a closed tube at 200#{176}C for 1.5 h (for RNA) or at 150#{176}C for 2.0 h (for DNA). The trifluoroacetic acid and formic acid are evaporated from the hydrolysates on a ...

Functional human globin messenger RNA but failed to stimulate the synthesis of any was isolated from reticulocytes of two paf. -chains, even though nonthalassemic tients with homozygous /3#{176}-thalassemia, human globin mRNA preparations conthree patients with sickle cell fl#{176}-thalassistently stimulated two to four times as semia, and one patient doubly heteromuch fl or globin chain synthe...

Campylobacter jejuni CG8486, which belongs to the HS4 complex, was isolated from a patient with inflammatory diarrhea in Thailand. This strain caused a diarrheal disease in ferrets comparable to that caused by C. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. Complete genome sequencing of CG8486 revealed a 1.65-Mb genome that was very similar to t...