Ubiquitination, the crucial posttranslational modification that regulates the eukaryotic proteome, is carried out by a trio of enzymes, known as E1 [ubiquitin (Ub)-activating enzyme], E2 (Ub-conjugating enzyme), and E3 (Ub ligase). Although most E2s can work with any of the three mechanistically distinct classes of E3s, the E2 UBCH7 is unable to function with really interesting new gene (RING)-...

Ubc13, a ubiquitin-conjugating enzyme (Ubc), requires the presence of a Ubc variant (Uev) for polyubiquitination. Uevs, although resembling Ubc in sequence and structure, lack the active site cysteine residue and are catalytically inactive. The yeast Uev (Mms2) incites noncanonical Lys63-linked polyubiquitination by Ubc13, whereas the increased diversity of Uevs in higher eukaryotes suggests an...

Seismic screening, evaluation, and rehabilitation design of two existing wood-framed structures are performed using FEMA 154, FEMA 356, ASCE/SEI 31, and the 1997 UBC. FEMA 154 screening demonstrates the importance of examining construction drawings. Plans revealed a structural deficiency, but screening alone indicated no further evaluation is needed. Demand-to-capacity ratios for shear walls an...

BRUCE is implicated in the regulation of DNA double-strand break response to preserve genome stability. It acts as a scaffold to tether USP8 and BRIT1, together they form a nuclear BRUCE-USP8-BRIT1 complex, where BRUCE holds K63-ubiquitinated BRIT1 from access to DSB in unstressed cells. Following DSB induction, BRUCE promotes USP8 mediated deubiquitination of BRIT1, a prerequisite for BRIT1 to...

Urinary bladder cancer (UBC) is one of the most common urological cancer types. Muscle invasive bladder cancer possesses high propensity for metastasis with poor prognosis. Honokiol is a lignan isolated from Magnolia officinalis with high bioavailability and potent anticancer effects. The results of the present study demonstrated that honokiol significantly inhibited UBC cell migration and inva...

OBJECTIVE There is increasing interest in patients with metachronous (MBC) and synchronous breast cancer (SBC). The objective of this study was to evaluate the occurrence and outcome of MBCs and SBCs. METHODS A retrospective study on women operated in our department for breast cancer between 2002 and 2005 was carried out. Patients were divided into three groups: women with MBC, SBC, and unila...

1 Dept. of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA; 2 Depts. of Biology and Chemistry, Syracuse University, Syracuse, NY 13244, USA; 3 Institut des Sciences Analytiques, UMR5280-Université de Lyon, 69100 Villeurbanne, France; 4 Dept. of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA; 5 NIST Center for Neutron Research, National Insti...

VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2.6 A resolution, revealing that determinants for ubiquitin recognition are conserved in nearly all VHS domains. VHS domains from all classes of VHS-domain containing proteins in yeast and humans, including ...

Background: Surgical complications after liver transplantation are known to contribute to morbidity and mortality. Evaluation of complication rates is a crucial part of assessing transplant program performance and identifying areas for improvement. A study was proposed to determine complication rates and outcomes using BC Transplant data. Methods: Patients undergoing deceased-donor liver transp...

In the last few years, susceptibility loci have been identified for urinary bladder cancer (UBC) through candidate-gene and genome-wide association studies. Prognostic relevance of most of these loci is yet unknown. In this study, we used data of the Nijmegen Bladder Cancer Study (NBCS) to perform a comprehensive evaluation of the prognostic relevance of all confirmed UBC susceptibility loci. D...