A new serological technique of diagnosing human hydatid disease, employing polystyrene latex particles coated with hydatid cyst fluid, and a modified technique using bentonite particles are described. Comparison of the latex test with the complement-fixation test performed on 102 sera has shown a total agreement of 98% and an agreement of 93.1% in the positive specimens. The bentonite test and ...

Complement plays a major role in inflammation and thrombosis associated with systemic lupus erythematosus (SLE) and the antiphospholipid syndrome (APS). A cross-sectional retrospective analysis was performed to evaluate serum complement fixation on platelets and thrombotic incidence using banked sera and clinical data from patients with SLE (n = 91), SLE with antiphospholipid antibodies (aPL) o...

A technique has been described for the demonstration of a human complement component by an immunofluorescent method. The component detected is beta(1C)-globulin, a moiety of the third complement component, which has previously been obtained in pure form and to which a specific antiserum has been prepared. It has been shown in a model system that the binding of beta(1C)-globulin as shown by immu...

The immunodiffusion and complement fixation tests for histoplasmosis were compared on 2,090 initial sera from 70 proven cases of histoplasmosis, 177 routine chest hospital patients, and 1,843 coal miners from outside the major endemic area for histoplasmosis. The complement fixation test using histoplasmin as antigen detected antibodies in the sera of 72.8% of the 70 proven cases, while the com...

Antigen from purified Mycoplasma pneumoniae organisms treated with Tween-80-ether was used in a solid phase enzyme immunoassay and compared with the conventional lipid containing complement fixation antigen for measuring antibodies in sera from patients with aseptic or bacterial meningitis or with apparent M pneumoniae infection. In immunoblotting of the enzyme immunoassay antigen, enzyme immun...

1. Sensitization confers upon the red cell the property of adsorbing complement from solution. The submicroscopic film of immune serum protein deposited upon the cell surface during sensitization, and completely analogous to the precipitate formed in a soluble antigen-antibody reaction (e.g., sheep serum vs. rabbit anti-sheep serum) acts as absorbent, the degree of sensitization (size of the fi...

A large proportion of high titre Coxsackie B5 complement-fixation tests was detected among sera giving false positive Wassermann reactions during 1969. Echo virus type 9 proved to be the prevailing enterovirus and is one of those which induces the formation of antibody to Coxsackie B5. One case of Echo virus type 9 meningitis is presented which produced a false positive Wassermann reaction and ...

containing a large amount of neutral sugars showed no complement fixation inhibition. while a O.5N HCI eluate containing proteins alone and one containing proteins and hexosamine showed a slight complenient fixation inhibition, The remaval of lipids from the type specific antigen by hydrolyzing with 1 N HCI for 4 minutes, followed by dialysis, revealed a very strong complement fixation inhibiti...

vorax strain V,. Serological relationships were determined by three procedures: immobilization, agglutination, and complement fixation. Antigenic relationships in general were dependent upon the method employed. The immobilization test provided the best method for uncovering these differences. The complement fixation test did not provide satisfactory identification of the different species.

Two methods of performing serological screening tests for syphilis are compared. One consisted of the Venereal Diseases Reference Laboratory (VDRL) slide test, the cardiolipin Wassermann reaction (CWR), and the Reiter protein complement fixation test (RPCFT) performed manually; the other was a fully automated system using two Technicon AutoAnalyzers (AAII), one for the automated reagin test (AR...