Zebrafish encodes several sialidases belonging to the NEU3 group, plasma membrane-associated member of family with high specificity toward ganglioside substrates. Neu3.1, Neu3.2 and Neu 3.3 have been expressed in E. coli purified using pGEX-2T expression system. Although all enzymes are by bacterial cells, Neu3.1 formed insoluble aggregates that hampered its purification. Neu3.3 oligomers as de...

Zebrafish (Danio rerio) is a teleost fish, common in home aquarium. This animal has emerged as a powerful model organism for studies of vertebrate development, diseases, biological pathways, toxicology mechanisms and organ regeneration. The adult teleost fish has the capacity to regenerate several organs, including their appendages, optic nerve, heart, spinal cord. These organs display poor reg...

T-box genes are conserved in all animal species. We have identified two members of the T-box gene family from the zebrafish, Danio rerio. Zf-tbr1 and zf-tbx3 share high amino acid identity with human, murine, chick and Xenopus orthologs and are expressed in specific regions during zebrafish development.

We have identified a novel Sox gene in zebrafish (Danio rerio), Sox31, closely related to mammalian group B Sox genes. The gene is maternally expressed. Zygotic transcription starts at gastrulation, in the presumptive neuroectoderm. Later, expression is restricted to the developing central nervous system, including forebrain, midbrain, hindbrain and spinal cord.

Differential transcriptional modulation of duplicated fatty acid-binding protein genes by dietary fatty acids in zebrafish (Danio rerio): evidence for subfunctionalization and neofunctionalization of duplicated genes BMC Evolutionary Biology 2009, 9:219 doi:10.1186/1471-2148-9-219 Santhosh Karanth ([email protected]) Santosh P Lall ([email protected]) Eileen M Denovan-Wright (emdenova@d...

Here, we report the draft genome sequences of Edwardsiella ictaluri strains LADL11-100 and LADL11-194, two isolates from natural outbreaks of edwardsiellosis in the zebrafish Danio rerio, as well as the sequences of the plasmids carried by the zebrafish strain of E. ictaluri.

Joint toxicity of arsenate and perchlorate was tested in larvae of Danio rerio. Results indicated that the 96-h median lethal concentrations of sodium arsenate and sodium perchlorate were 258.8 and 1,401.2 mg/L, respectively, and that arsenate and perchlorate generally showed a concentration-additive effect.

We have developed a bioinformatics pipeline for the comparative evolutionary analysis of Ensembl genomes and have used it to analyze the introns of the five available teleost fish genomes. We show our pipeline to be a powerful tool for revealing variation between genomes that may otherwise be overlooked with simple summary statistics. We identify that the zebrafish, Danio rerio, has an unusual ...

During embryonic development, a complex organism is formed from a single starting cell. These processes of growth and differentiation are driven by large transcriptional changes, which are following the expression and activity of transcription factors (TFs). This study sought to compare TF expression during embryonic development in a diverse group of metazoan animals: representatives of vertebr...

Melanopsin has been implicated in the mammalian photoentrainment by blue light. This photopigment, which maximally absorbs light at wavelengths between 470 and 480 nm depending on the species, is found in the retina of all classes of vertebrates so far studied. In mammals, melanopsin activation triggers a signaling pathway which resets the circadian clock in the suprachiasmatic nucleus (SCN). U...