The present study was designed to test the efficacy, as assessed by blastocyst production, of culturing slaughterhouse-derived bovine oocytes in Ménézo's B2 (B2) medium with or without serum and with or without buffalo rat cell (BRL) coculture. In addition, OPU-derived oocytes were cultured in B2-BRL coculture with or without serum for the first 72 h or TCM 199-BRL coculture with serum and the ...

The nucleolus is required for ribosome biogenesis. Human cells have 2 or 3 nucleoli associated with nucleolar organizer region (NOR)-bearing chromosomes. An increase in number and altered nucleolar morphology define cancer cells. However, the mechanisms that modulate nucleolar morphology and function are unclear. Here we show that in addition to localizing at the nuclear envelope, lamin B2 loca...

6,798,120 B1 9/2004 Fearing et al. 7,267,150 B2 * 9/2007 Shimizu et a1. ............. .. 156/378 7,446,423 B2 * 11/2008 Sakai et a1. ...... .. 257/783 7,678,211 B2 * 3/2010 Takasaki et a1. 156/64 7,896,047 B2 * 3/2011 Yamamoto ....... .. 156/378 7,988,803 B2 * 8/2011 Takasaki et a1. ....... .. 156/64 8,091,764 B2 * 1/2012 Suga et a1. ............. .. 228/18021 2006/0264086 A1 11/2006 Nolan 2008...

Ephrin-B2 is predominately expressed in endothelium of arterial origin, involved in developmental angiogenesis and neovasculature formation through its interaction with EphB4. Despite its importance in physiology and pathological conditions, it has been challenging to produce monoclonal antibodies against ephrin-B2 due to its high conservation in sequence throughout human and rodents. Using a n...

The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells from spleen, tonsil, or peripheral blood were stimulated in vitro with pokewe...