Although much research has been conducted, the pattern of microsatellite distribution has remained ambiguous, and the development/utilization of microsatellite markers has still been limited/inefficient in Brassica, due to the lack of genome sequences. In view of this, we conducted genome-wide microsatellite characterization and marker development in three recently sequenced Brassica crops: Bra...

INTRODUCTION Brassica vegetables have been related to the prevention of cancer and degenerative diseases, owing to their glucosinolate and phenolic content. OBJECTIVE Identification of glucosinolates, flavonoids and hydroxycinnamic acids in representative varieties of kale, cabbage and leaf rape. METHODOLOGY One local variety of each crop was evaluated in this study using a multi-purpose ch...

The cabbage maggot, Delia radicum (L.) is an important insect pest of eruciferous crops in upstate New York. This species causes considerable damage to seedlings and young plants by feeding on roots and stems, resulting in plant stand loss and yield loss. Five crucifer accessions (Brassica oleracea variety italica L.,'Green Comet'; B. oleracea L.,'Rapid Cycling' [Crucifer Genetics Cooperative 3...

Two proteins which have carbonate dehydratase (carbonic anhydrase, EC 4.2.1.1) activity were shown to be in the chloroplasts and in the cytosol of leaves of Brassica chinensis, Spinacia oleracea, and in variegated leaves of Tradescantia albiflora and Hedera canariensis. The chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. It was se...

Streptomycin enhances the synthesis of anthocyanins and inhibits the synthesis of chlorophylls and the development of chloroplasts in dark-grown seedlings of cabbage (Brassica oleracea), mustard (Sinapis alba), tomato (Lycopersicon esculentum), and turnip (Brassica rapa) exposed to prolonged periods of irradiation in various spectral regions. These results suggest that the contribution of photo...

The Resistance to Powdery Mildew 8 (RPW8) locus confers broad-spectrum resistance to powdery mildew in Arabidopsis thaliana. There are four Homologous to RPW8s (BrHRs) in Brassica rapa and three in Brassica oleracea (BoHRs). Brassica napus (Bn) is derived from diploidization of a hybrid between B. rapa and B. oleracea, thus should have seven homologs of RPW8 (BnHRs). It is unclear whether these...

Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ...

BACKGROUND AND AIMS Plants damaged by herbivores emit a variety of volatile organic compounds (VOCs). Here we used proton-transfer reaction mass spectrometry (PTR-MS) as a sensitive detection method for online analysis of herbivore-induced VOCs. Previously, it was found that Brassica nigra plants emit several sulfur-containing VOCs when attacked by cabbage root fly (Delia radicum) larvae with m...

We describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derived from both genetically mapped DNA markers flan...

chlormequat (cycocel or ccc), the plant growth retardant, was evaluated for its ability to control plant height in brassica oleracea cultivar ‘kamome white’ and ‘nagoya red’. different concentrations of ccc (0, 500, 1000 and 1500 mg/l) were sprayed and drenched on plants 40 days after transplanting. data were recorded the 60 and 90 days after transplanting. the 1500 mg/l of ccc resulted in abou...