Drugs and solutions All tissues were perfused and maintained with KRB solution containing (mmol/liter): 120.35 NaCl, 15.5 NaHCO3, 5.9 KCl, 1.2 MgCl2, 1.2 NaH2PO4, 2.5 CaCl2, and 11.5 glucose. The KRB solution warmed to a physiological temperature of 37 ± 0.2°C and bubbled with a mixture of 97% O2–3% CO2. For experiments using external solutions with zero [Ca]o, CaCl2 was omitted and 1 mM EGTA w...

BACKGROUND Normal ionic perfusate containing butanedione monoxime (BDM), a reversible myofilament inhibitor, could be better than either a high potassium (KCl) or a low calcium (CaCl2) perfusate for long-term cardiac preservation. This hypothesis was tested in 70 isolated guinea pig hearts. METHODS AND RESULTS Three groups--time control (8 hours, 37 degrees C), cold control (22 hours, 3.8 deg...

Insulinomas are β-cell tumors that cause hypoglycemia through inappropriate secretion of insulin. Characterization of the in vitro dynamics of insulin secretion by perifused fragments of 10 human insulinomas permitted their subdivision into three functional groups with similar insulin content. Group A (four patients with fasting and/or postprandial hypoglycemic episodes) showed qualitatively no...

The main objective of this work was to assess the effectiveness of salicylic acid (SA), calcium chloride (CaCl2) or sodium bicarbonate (NaHCO3), and packaging on some qualitative properties of apricot fruit during cold storage. The experiments were conducted using a completely randomized design as factorial, with three replicates. Fruits were dipped in SA (0.1 or 0.5 mM), CaCl2 (1 or 2%) or NaH...

BACKGROUND The purpose of this study was to introduce a novel, simple and effective technique for creating a reliable rabbit model of abdominal aortic aneurysm (AAA) via a combination of periaortic calcium chloride (CaCl2) and elastase incubation. METHODS Forty-eight New Zealand white rabbits were divided into four groups. The AAA model was developed via a 20-minute periaortic incubation of C...

Microstructure development and the kinetics of hydration of pure tricalcium silicate (C3S) and CaCl2-accelerated C3S pastes were investigated by performing isothermal calorimetry and in situ small-angle neutron scattering (SANS) measurements on parallel specimens during the first few days of hydration, as well as on 28-day old specimens hydrated under the same curing conditions (water/cement ra...

The H NMR relaxometry in combination with water proton spin–spin relaxation time measurements and rheometry have been applied to study the ionic gelation of 1% w/w aqueous low methoxyl pectin solution induced by divalent Ca cations from a calcium chloride solution. The model-free approach to the analysis of H NMR relaxometry data has been used to separate the information on the static (β) and d...

INTRODUCTION This in vitro study investigated the effect of adding 10% calcium chloride (CaCl2) on push out bond strength of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA) to root canal dentin. METHODS AND MATERIALS A total of 120 root dentin slices with 2 mm thickness were prepared from sixty single-rooted human teeth. Dentinal discs were enlarged to achieve 1.3 m...

The postharvest quality of tomato fruit harvested at the pink stage and dipped in 3 different concentrations of CaCl2 for different dip times (30, 20 and 10 minutes) were studied. Tomato fruits dipped in 6% CaCl2 for 30 and 20 minutes retained significantly (P < 0.05) higher level of firmness, titrable acidity and vitamin C than fruits dipped in 2% CaCl2 and the control. The calcium chloride tr...

The effects of temperature and calcium on pectin methylesterase (PME) activity and texture in tomato pericarp material were examined. Heating thin slices of pericarp to temperatures between 50 °C and 75 °C led to the rapid evolution of methanol from the material, indicating an activation of PME. This activity was further stimulated when CaCl2 (up to 2.0% w/v) was added. When applied to half-inc...