The capsid proteins VP2 and VP3 of infectious bursal disease virus, a birnavirus, are derived from the processing of a large polyprotein: NH2-pVP2-VP4-VP3-COOH. Although the primary cleavage sites at the pVP2-VP4 and VP4-VP3 junctions have been identified, the proteolytic cascade involved in the processing of this polyprotein is not yet fully understood, particularly the maturation of pVP2. By ...

The carboxy terminus of GLUT-4 contains a functional internalization motif (Leu-489Leu-490) that helps maintain its intracellular distribution in basal adipocytes. This motif is flanked by the major phosphorylation site in this protein (Ser-488), which may play a role in regulating GLUT-4 trafficking in adipocytes. In the present study, the targeting of GLUT-4 in which Ser-488 has been mutated ...

Supplemental Experimental Procedures Confocal UV Laser Scanning Microscopy of Filipin-Sterol Fluorescence Dot-Blot Detection of Filipin-Sterol Fluorescence Analysis of Arabidopsis roots was performed by employing a Nikon Sterols (Sigma) and 4-cholesten-3-one (Sigma) in chloroform at 20 Diaphot 300 inverted microscope, connected to a Nikon RCM 8000 mM were spotted onto Transblot nitrocellulose m...

Serum amyloid A protein (SAA) is an acute-phase reactant, known to mediate pro-inflammatory cellular responses. This study reports that CLA-1 (CD36 and LIMPII Analogous-1; human orthologue of the Scavenger Receptor Class B Type I (SR-BI)) mediates SAA uptake and downstream SAA signaling. Flow cytometry experiments revealed more than a 5-fold increase of Alexa-488 SAA uptake in HeLa cells stably...

PURPOSE To investigate the significance of slowly rising abdominal pressure (SRAP), which is often observed in nonneurogenic children during bladder filling in video urodynamic studies (VUDSs). METHODS The records of patients who underwent VUDS from July 2011 to June 2013 were reviewed. SRAP was defined as a rising curve over 5 cm H2O from the baseline abdominal pressure during the filling ph...

Steady state LIF spectra using 488 nm of cw Ar + laser and 355 nm of pulsed Nd:YAG laser, fluorescence induction kinetics using 488 nm laser light were studied in wheat seedlings exposed to increasing cadmium (Cd) concentrations (0.01, 0.1, 1.0 and 2.0 mM). In addition, some growth parameters and pigment content were also measured. The LIF spectra of the leaves exhibited two characteristic band...