نتایج جستجو برای: 100 blood samples
تعداد نتایج: 1384114 فیلتر نتایج به سال:
We have developed a novel blood lysis-centrifugation approach for highly sensitive Mycobacterium tuberculosis detection in large volumes of blood with the Xpert MTB/RIF assay. One through 20 ml of blood was spiked with 0.25 to 10 CFU/ml of the M. tuberculosis surrogate M. bovis BCG. Multiple replicates of each sample were processed by a new lysis-centrifugation method and tested with the Xpert ...
An automated fluorometric procedure, using AutoAnalyzer modules and based on the alcohol dehydrogenase reaction, has been developed for the determination of ethanol in fingertip capillary blood samples. Whole blood is measured directly from the finger prick into 0.02-mi. capacity automatic-filling disposable micropipets. The sample is quickly discharged into an AutoAnalyzer cup containing 1.0 m...
OBJECTIVE To validate and develop an immunonephelometric assay for the determination of alpha-1 antitrypsin (AAT) levels in dried blood spots from COPD patients in Brazil. METHODS We determined AAT levels in serum samples and dried blood spots from 192 COPD patients. For the preparation of dried blood spots, a disk (diameter, 6 mm) was placed into a tube, eluted with 200 µL of PBS, and stored...
An immunofluorometric sandwich assay for determination of S-100 protein in cerebrospinal fluid (CSF) and blood is described. The lower detection limit was 0.015 micrograms/l of S-100 protein. Intra-assay and inter-assay imprecision (coefficients of variation, CVs) were 2.1 to 3.2% and 7.8 to 11.6%, respectively. S-100 protein recovery in cerebrospinal fluid was 94 to 103%. In blood the recovery...
OBJECTIVE To evaluate the potential effects of a 308-km ultra-marathon on bone and cartilage biomarkers. METHOD Venous blood samples were collected at pre-race, 100 km, 200 km, and 308 km checkpoints. The following markers of cartilage damage and bone metabolism were studied: osteocalcin (OC), osteoprotegerin (OPG), and calcium, phosphorous, and cartilage oligomeric matrix protein (COMP). R...
cause mild overtreatment, i.e., a reduction in dietary Phe allowances in excess of metabolic tolerance. This is not critical with Phe concentrations at or above the upper limit of normal. Still, neurological outcome has also been demonstrated to be adversely affected by Phe deficiency (14 ). In view of an overestimation by up to 60 mmol/L in the low concentration range and a target range of 40–...
Voluntary blood donors are people who voluntarily donate their to other and not certain patients. Before is given the recipient, a filter test or screening carried out. A process of separating that can be transfused infected with an infectious disease. Research has been conducted title Infectious Disease Screening Results Hemoglobin (Hb) Levels in Donors at Indonesian Red Cross Blood Transfusio...
OBJECTIVE To estimate performance of a single-nucleotide polymorphism-based noninvasive prenatal screen for fetal aneuploidy in high-risk and low-risk populations on single venopuncture. METHODS One thousand sixty-four maternal blood samples from 7 weeks of gestation and beyond were included; 1,051 were within specifications and 518 (49.3%) were low risk. Cell-free DNA was amplified, sequence...
leptospirosis is one of the zoonotic diseases caused by different serovars of the species leptospira interrogans . the source of infection in humans is usually either direct or indirect contact with the urine of infected animals. in this study, 400 human blood samples collected from tribal areas of farsan and koohrang cities in west centeral iran. the serum samples were analyzed employing mi...
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