A reference radioassay for vitamin B12 in serum, in which human serum is used as binder and DEAEcellulose is used to separate free and bound B12, was compared (194 sera) with a packaged kit (“Phadebas B12 Test”), in which intrinsic factor is used as binder and poiysaccharide for separation. The correlation coefficient was 0.949. With the kit, added B12 was identifiable, proportional dilution ch...

Pernicious anemia is a common cause of vitamin B12 deficiency. Here, we discuss a case of a young woman who presented with severe anemia along with a history of iron deficiency anemia. After a review of her clinical presentation and laboratory data, we identified an autoimmune hemolytic anemia and a concomitant pernicious anemia. The concurrence of both these hematological diagnoses in a patien...

BACKGROUND The AT1 receptor has been implicated in the pathogenesis of hypertension and atherosclerosis. Estrogen deficiency is also associated with cardiovascular diseases. Therefore, we examined the AT1 receptor gene expression in ovariectomized rats with and without estrogen replacement therapy and the influence of estrogen on AT1 receptor expression in cultured vascular smooth muscle cells....

Many children in low- and middle-income countries may have inadequate intake of vitamin B12 and folate; data confirming these inadequacies are limited. We used biochemical, demographic, behavioral and anthropometric data to describe the folate and vitamin B12 concentrations among six- to 23-month-old Nepalese children. Vitamin B12 (serum B12 < 150 pmol/L) and folate deficiencies (red blood cell...

OBJECTIVES To associate the severity of nutritional anaemia with serum levels of ferritin, vitamin B12 and folate; and to determine demographic, socio-economic and nutritional correlates for nutritional anemia in adolescents. METHODS Cross-sectional hospital-based study among 200 adolescents (10-18 y) with anemia. Dietary intake (24-h recall), and serum levels of folate, vitamin B12 and ferri...

vitamin B12. Therefore, the results of the study of Sirdah et al. Iron is themost abundant heavymetal in the earth’s crust and is required by almost all organisms.1 In the human body iron is required by and present in all cells and has several vital functions.2 Anemia due to iron deficiency is the commonest type of nutritional anemia worldwide, and occurs when body iron stores become inadequate...

BACKGROUND Cobalamin deficiency is responsible for hematological, neurological, neurocognitive, and neuropsychiatric impairments and is a risk factor for cardiovascular diseases, particularly in the elderly people. METHODS In order to determine B12 status in old inpatients, a total number of 14,904 hospitalized patients in whom B12 measurements were performed in five hospitals in the Paris me...

OBJECTIVE To describe the prevalence of iron, folate, and B12 deficiencies in Mexican women of reproductive age from the National Health and Nutrition Survey (Ensanut) 2012. MATERIALS AND METHODS Data came from a national probabilistic survey, representative from rural and urban areas, and different age groups. Blood samples were obtained from 4 263, 20 to 49 years old women for serum ferriti...

Production of interferon gamma (IFN-gamma) is critical for optimal antitumor immunotherapy in several preclinical animal models. Interleukin-12 (IL-12)-induced IFN-gamma production is markedly defective after autologous stem cell transplantation. Quantitative deficiency in CD4 T cells, relative increase in CD25+CD4+ T cells, and bias toward T helper 2 (Th2) differentiation are not the primary m...

Populations with special physiological state, such as pregnant, nursing and women of child-bearing age, have been identified as nutritionally vulnerable during natural disaster. The objective of this survey was to evaluate the prevalence of anaemia and micronutrient status of women of reproductive age in April 2009 one year after the Wenchuan Earthquake. The survey recruited 58 pregnant, 66 lac...