In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant...

The use of quantitative structure–activity relationships, since its advent, has become increasingly helpful in understanding many aspects of biochemical interactions in drug research. This approach was utilized to explain the relationship of structure with biological activity of antibacterial. For the development of new fungicides against, the quantitative structural–activity relationship (QSAR...

The present synthetic strategy involves the synthesis of indolyl-triazolo-thiadiazole heterocyclic ring systems 8–13 from condensation 4-amino-5-(1H-indol-2-yl)-3H-1,2,4-triazole-3-thione 1 with aromatic carboxylic acid derivatives 2–7 in presence POCl3 for h. All compounds were obtained very good yields and have been well-characterized using spectroscopic techniques. Exclusively, quality cryst...