Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...

The organization of 1-butanol and 1-hexanol at the air-liquid interface of aqueous, aqueous ammonium bisulfate, and sulfuric acid solutions was investigated using vibrational broad bandwidth sum frequency generation spectroscopy. There is spectroscopic evidence supporting the formation of centrosymmetric structures at the surface of pure butanol and pure hexanol. At aqueous, ammonium bisulfate,...

Thin layer chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GC-MS) techniques are proposed for qualitative analysis of lubricating oil additives (Hitec 2915). The additives were dissolved in heptanes, extracted with mixed solvents heptane: acetonitrile (3:2) and spotted on TLC paper. The best combination solvents as developer for TLC analysis were carbon tetrachloride: heptanes (9...