A (25)Mg(2+)-operated hyper-activation of ATP synthesis has been investigated in mitochondria (Mt) isolated from iron-rich and iron-poor rat tissues: spleen, liver, skeletal muscle, myocardium, kidneys, brain. Both magnetic ((25)Mg) and non-magnetic ((24)Mg) magnesium isotopes were separately administered to estimate the degree of the ATP production related to the magnetic isotope effect (MIE) ...

Gas-phase multinuclear magnetic resonance (MR) spectra allow the total elimination of intermolecular effects from magnetic shielding in molecules. Consequently, the resonance frequencies of isolated atoms and molecules are also available for reading from MR spectra. Following the connectivity between nuclear magnetic moments, resonance frequencies of two different nuclei in the same MR experime...

Isotope separation is one of the grand challenges of modern society and holds great potential for basic science, medicine, energy, and defense. We consider here a new and general approach to isotope separation. The method is based on an irreversible change of the mass-to-magnetic moment ratio of a particular isotope in an atomic beam, followed by a magnetic multipole whose gradients deflect and...

A combined experimental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase reaction is described. The experimental studies focus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed in these reactions is an intrinsic isotope effect resulting from a single rate-limiting step or is a composite of multiple rate-limit...

Literature data on H /D isotope effects on polarizability are reviewed. An argument based on perturbation theory correlates the isotopic differences with electronic transition moments via vibra­ tional averaging. The contribution of the second moment isotope effect is especially important. The approach is first illustrated with a calculation on H 2/D 2 and then employed to correlate differentia...

Background: Angiotensin converting enzyme (ACE) is the key enzyme, regulates blood pressure which encoded by 21kb gene that consists of 26 exons and located on chromosome 17, contains a polymorphism in form either Insertion (I) or Deletion (D). The aim was to study effect antihypertensive drugs patients essential hypertension associated with ACE polymorphism. Methods: Hypertensive were recruite...

The D/H ratios of hydrogen gas in equilibrium with aqueous alkali halide solutions were determined at 25 °C, using a hydrophobic platinum catalyst. The hydrogen isotope effect between the solution and pure water changes linearly with the molality of the solution at low concentrations, but deviates from this linearity at higher concentration for all alkali halide solutions. The magnitude of the ...

The diffusional isotope effect of a monatomic Lennard-Jones liquid is calculated by molecular-dynamics simulation. Mass differences of 10-40 % were used. At equilibrium density, with decreasing temperature a strong reduction of the isotope effect is found that indicates a marked increase of the collectivity of motion. Changing the density at constant temperature, the same effect is seen that sh...

Three-dimensional, triple resonance NMR techniques are described for measurement of two-bond (intraresidual) and three-bond (sequential) amide deuterium isotope effects on 13CR chemical shifts. Measurements were carried out for uniformly 15N and 13C labeled human ubiquitin equilibrated in a 50% H2O/50% D2O mixed solvent. The three-bond isotope shift, 3∆CR(ND), ranges from about 10-50 ppb, and, ...

Chlorinated ethenes are prevalent groundwater contaminants. To better constrain (bio)chemical reaction mechanisms of reductive dechlorination, the position-specificity of reductive trichloroethene (TCE) dehalogenation was investigated. Selective biotransformation reactions (i) of tetrachloroethene (PCE) to TCE in cultures of Desulfitobacterium sp. strain Viet1; and (ii) of TCE to cis-1,2-dichlo...