Recently about 500 new cases of leprosy have been reported each year in Thailand. In addition to a steady rate of new case detection, Thailand is in Southeast Asia where leprosy is endemic in neighbouring countries; therefore, strain differentiation could be useful in tracing origins and routes of infection, and general leprosy surveillance. To identify suitable markers for differentiation of M...

PPARã, the gamma isoform of a family of peroxisome proliferator activated receptors, plays a key role in adipocyte diVerentiation. Recently, its broad expression in multiple tissues and several epithelial cancers has been shown. Further, somatic loss of function mutations in PPARã have been found in primary colorectal carcinomas. We sought to determine if somatic high penetrance mutations in th...

By molecular genetic approach using polymorphic DNA markers which detect allelic deletion at specific chromosomal loci, we analyzed 30 human stomach cancers for possible loss of chromosomal heterozygosity. We analyzed 25 loci on 18 different chromosomes covering regions frequently deleted in several types of cancers. Loss of chromosomal heterozygosity was observed only in five of 30 cases exami...

PREMISE OF THE STUDY Ficinia spiralis (Cyperaceae) is a declining sand-binding sedge of ecological and cultural importance. Microsatellite primers were developed in F. spiralis to investigate how population genetic structure is related to the pronounced morphological, physiological, and ecological variation observed in this species. METHODS AND RESULTS A 454 shotgun-sequencing approach was us...

Four Saudi Arabian indigenous sheep populations including Najdi, Hbsi, Arb, and Naemi were genotyped for 16 microsatellite markers recommended by the food and agriculture organization (FAO). This study aims to provide information on the genetic structure of the breeds analyzed and give indications and proposals for the cross breeding program. All loci were polymorphic in all populations and loc...

The study of the extent and pattern of genetic variation of six populations of Azadirachta excelsa (Jacks) Jacobs, i.e. Manong (Perak), Pokok Sena (Kedah), Sik (Kedah), FRIM (Selangor), Merchang (Terengganu) and Sg. Caru (Pahang) was carried out using starch gel electrophoresis. The analysis on eleven enzyme systems was found to be coded by 25 and 26 loci. The mean of observed heterozygosity ra...

UNLABELLED • PREMISE OF THE STUDY The development of microsatellite primers in the endangered species Dracaena cambodiana will be the foundation for genetic and conservation studies of D. cambodiana and several Dracaena species. • METHODS AND RESULTS A total of 26 microsatellite markers were developed in Chinese populations of D. cambodiana, using the Fast Isolation by AFLP of Sequences Con...

Human Xq28 is highly gene dense with over 27 loci. Because most of these genes have been mapped by linkage to polymorphic loci, only one of which (DXS52) is informative in most families, a search was conducted for new, highly polymorphic Xq28 markers. From a cosmid library constructed using a somatic cell hybrid containing human Xq27.3----qter as the sole human DNA, a human-insert cosmid (c346)...

We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a s...

A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 p...