We describe several two-dimensional NMR experiments to facilitate the analysis of overcrowded proton-decoupled deuterium spectra of chiral molecules oriented in poly-γ-benzyl-L-glutamate liquid crystalline solutions. These 2D NMR experiments allow the correlation between the components of each quadrupolar doublet in the deuterium spectra and then their assignment on the basis of chemical shift....

Lignin changes during plant growth were investigated in a selected Eucalyptus globulus clone. The lignin composition and structure were studied in situ by a new procedure enabling the acquisition of two-dimensional nuclear magnetic resonance (2D-NMR) spectra on wood gels formed in the NMR tube as well as by analytical pyrolysis-gas chromatography-mass spectrometry. In addition, milled-wood lign...

Melanoma is the most aggressive type of skin cancer and efforts to improve the diagnosis of this neoplasia are largely based on the use of cell lines. Metabolomics is currently undergoing great advancements towards its use to screening for disease biomarkers. Although NMR metabolomics includes both 1D and 2D methodologies, there is a lack of data in the literature regarding heteronuclear 2D NMR...

Among the different forms of spectroscopy, nuclear magnetic resonance (NMR) shows an intimate connection between theory and experiment. NMR therefore enjoys great popularity in the development of new methods. Following the rapid development in electronic and computer technology, NMR techniques evolved from singlechannel to multi-channel and multi-dimensional modes. The original continuous wave ...

The development of two dimensional Fourier transform NMR techniques by Professor Ernst during the Seventies and Eighties created a revolution in the study of biomolecules by NMR. The ubiquitous COSY experiment and its many variants, especially the DQFC and TOCSY experiments made it possible to obtain assignments of resonances of molecules containing several hundreds of protons a task which was ...

New approaches to the characterization of resonances in the solid-state NMR spectroscopy of half-integer quadrupolar nuclei are explored, on the basis of the acquisition of heteronuclear separate-local-field spectra on rotating solids. In their two-dimensional version, these experiments correlate for each chemical site a second-order quadrupolar MAS powder pattern with the dipolar MAS sideband ...

A new protocol for acquiring multidimensional NMR spectra within a single scan is introduced and illustrated. The approach relies on applying a pair of frequency-chirped excitation and storage pulses in combination with echoing magnetic field gradients, in order to impart the kind of linear spatial encoding of the NMR interactions that is required by ultrafast 2D NMR spectroscopy. It is found t...

Single-scan 2D NMR relies on a spatial axis for encoding the indirect-domain internal spin interactions. Various strategies have been demonstrated for fulfilling the needs underlying this procedure. All such schemes use gradient-echoed sequences that leave at their conclusion solely the effects of the internal interactions along the indirect domain; they also include a real-time scheme that tho...