Facile and specific methods to quantify a nucleobase in biological samples are of great importance for diagnosing disorders in nucleic acid metabolism. In the present study, a novel fluorogenic reaction specific for uracil has been developed. The reaction was carried out in an alkaline medium containing benzamidoxime and K(3)[Fe(CN)(6)] which were heated for 2.0 min. Under the optimum reaction ...

Pseudomonas aeruginosa is an ubiquitous, opportunistic pathogen whose biofilms are notoriously difficult to control. Here we discover uracil influences all three known quorum-sensing (QS) pathways of P. aeruginosa. By screening 5850 transposon mutants for altered biofilm formation, we identified seven uracil-related mutations that abolished biofilm formation. Whole-transcriptome studies showed ...

Baker’s yeast UMP:pyrophosphate phosphoribosyltransferase (EC 2.4.2.9) has been extensively purified and the final preparation seemed to be pure but not homogeneous. The native enzyme showed a molecular weight of 80,000 calculated by gel filtration; disc gel electrophoresis performed in the presence of sodium dodecyl sulfate and reducing agents showed two dissimilar subunits and the sum of thei...

The wobble hypothesis does not discriminate between uracil and thymine. Methylation could favor further stabilization of uracil in the keto form. Thymine is present in keto form only and can pair up but with adenine. Uracil can easily construct the enol form; that is why it forms the U-G pair. In previous E-print I proposed an alternative to the wobble hypothesis [1]. According to my reviewers ...

The syntheses of the title compounds are described. 1- [[2- Amino -1 - (hydroxymethyl) ethoxy] methyl] uracil exhibitslittleactivity against herpesviruses (HSV) in vivo

ICP4 is an important factor regulating the life cycle of HSV1. This conserved protein has several molecular functions, including activation of expression of viral late gene transcripts and inhibition of immediate early genes. Although ICP4 and its Alphaherpesvirinae homologs (eg.: IE62 of VZV) have been subjects of various molecular studies, a complete view of their molecular function is lackin...