An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash step...

BACKGROUND Adenosine has been suggested to play an important role in the regulation of renal function. We developed a simple and sensitive binding assay for the detection of adenosine based on the displacement of [(3)H]adenosine from S-adenosylhomocysteine (SAH) hydrolase in its reduced form. METHODS SAH hydrolase was purified to apparent homogeneity from bovine kidney by standard chromatogra...

Urinalysis is often interfered by vitamin C and PH. This study was to evaluate the interference of PH in urinalysis, minimize risk make effective communication. 732 leftover urine samples which concentration 0.0 mmol/L either test-strip results (Glucose, Blood, Bilirubin, Leucocyte, Nitrite, Protein, Ketone) positive that were collected. The test checked out using these samples. When chemical p...

We assessed the reproducibility, recovery, and stability of several circulating metabolites--glucose, pyruvate, lactate, alanine, glutamate, glutamine, 3-hydroxybutyrate, acetoacetate, and glycerol--in the presence of sulfosalicylic acid (SSA), which was used to deproteinize blood. The assays, which involved reactions linked to NADH/NAD+, were carried out at 37 degrees C and measured at 355 nm ...

Epoxy coatings incorporating different content of sulfosalicylic acid doped polyaniline (PANI-SSA) have been investigated for corrosion protection of 5083 aluminum alloy in 3.5% NaCl solution. The performance of the coatings is studied using a combination of electrochemical impedance spectroscopy (EIS), open circuit potential (OCP), gravimetric tests, adhesion tests, scanning electron microscop...

model for the study of neurotransmitter metabolism.'Therefore, we attempted to measure the concentration of glutamate and aspartate in platelets. We studied 12 healthy subjects ranging in age from 20 to 47 years (six men, six women). All were free of drugs known to affect platelet function for at least 2 weeks before the study. Blood (8 ml), drawn from an antecubital vein from subjects resting ...

A high-performance liquid chromatographic method was developed for the quantification of doxorubicin derived from PEGylated liposomal doxorubicin (Doxil) and its major metabolite in human plasma. This method utilizes Triton X-100 to disperse the liposome, followed by a protein precipitation step with 5-sulfosalicylic acid. Analytes in the resultant supernatant are separated on a Discovery RP am...

Simultaneous determination of FeII and FeIII mixtures by spectrophotometric methods is a difficult problem in analytical chemistry because of spectral interferences. By multivariate calibration methods, such as partial least squares (PLS), it is possible to obtain a model adjusted to the concentration values of the mixtures used in the calibration range. The method is based on developing the re...