PCR Conditions: The PCR reaction is carried out in a total volume of 50 /*1 containing approximately 250 ng DNA, 2 units Taq DNA polymerase, 50 pmol of each primer, 200 /M dNTP's, 10 mM Tris-HCl pH 8.3, 50 mM KC1, 1.5 mM MgCl2, 0.001% gelatin. The amplification is performed for 30 cycles with an annealing temperature of 60°C. The amplified product is digested with TaqI and the DNA fragments are...

Spergularia marina (Caryophyllaceae) is a halophytic species and widely distributed among the sea shores of Turkey. Its seeds maybe unwinged or winged. Laboratory experiments were conducted to determine the effects of salinity (0, 50, 100 and 500 mM NaCl) and temperature (10, 15, 25, 30, 35 degrees C) on seed germination. S. marina showed 73.3% germination in non-saline controls at 25 degrees C...

Zumkeller M, Behrmann R, Heissler HE, Dietz H. Computed tomographic criteria and survival rate for patients with acute subdural hematoma. Neurosurgery 1996;39: 708–713 Among 174 patients with acute subdural hematomas, hematoma thickness exceeding 18 mm was associated with survival rates that progressively fell below 50%. A 50% survival rate was reached when the midline shift reached 50%, and su...

Introduction Pavement texture is defined by the irregularities on a pavement surface that deviate from an ideal, perfectly flat surface. As shown in Figure 1, the World Road Association (PIARC) has established standard categories of texture, classified by wavelength. These categories include microtexture (wavelengths up to 0.5 mm), macrotexture (0.5 to 50 mm), megatexture (50 to 500 mm), and ro...

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0 10 20 30 −50 0 50 100 150 r (mm) U (m /s ) x/DB = 0.2 0 10 20 30 −10 −5 0 5 10 r (mm) V (m /s ) x/DB = 0.2 0 10 20 30 −50 0 50 100 150 r (mm) U (m /s ) x/DB = 0.6 0 10 20 30 −10 −5 0 5 10 r (mm) V (m /s ) x/DB = 0.6 0 10 20 30 −50 0 50 100 150 r (mm) U (m /s ) x/DB = 1.4 0 10 20 30 −10 −5 0 5 10 x/DB = 1.4 r (mm) V (m /s ) Experiment Raman et al. LES/HPDF Liu et al. Merci et al. Fig. 1. Mean ...

We investigated the ability of zinc sulfate (5, 25, 50 mM) to inhibit the sweetness of 12 chemically diverse sweeteners, which were all intensity matched to 300 mM sucrose [800 mM glucose, 475 mM fructose, 3.25 mM aspartame, 3.5 mM saccharin, 12 mM sodium cyclamate, 14 mM acesulfame-K, 1.04 M sorbitol, 0.629 mM sucralose, 0.375 mM neohesperidin dihydrochalcone (NHDC), 1.5 mM stevioside and 0.01...