A number of compounds have been reported to be specific inhibitors of protein kinases mediated by structural-based selectivity, but the development of specific inhibitors has not yet been addressed in plant science. Here we tested C2, C6, and N9-trisubstituted purines to determine the basic relationship between their chemical structure and inhibitory activity versus a plant mitogen-activated pr...

An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed ...

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population demand for forestry products results overexploitation forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change have available afforestation. For this reason, propagation methods should be improved ...

Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied evaluate shoot capacity whereas α-naphthalene acetic acid (NAA) with (BAP), 2, 4-dichlorophenoxyacetic (2, 4-D) were tested optimize root induction two peanut culti...

Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated ...

The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from ...

Abstract Kale ( Brassica oleracea convar. acephala var. sabellica ) is one of the oldest vegetable varieties with highest nutritional value and stress resistance. This work aimed to establish an efficient kale micropropagation protocol. Shoot tips were used as explants during axillary shoots multiplication. The most effective cytokinin (6 10 per explant after 1st 2nd passage, respectively) was ...