In order to analyze the roles of some repair genes in the processing of bleomycin-induced DNA damage and, especially, the interrelationships among the involved repair pathways, we investigated the potentially lethal effect of bleomycin on radiosensitive mutants of Saccharomyces cerevisiae defective in recombination, excision, and RAD6-dependent DNA repair. Using single, double, and triple rad m...

BACKGROUND A microwell modification of the bleomycin assay for determining non-transferrin-bound iron (NTBI) was evaluated and compared with a chelation method. METHODS The bleomycin assay reagent and sample volumes were halved, and measurements were done in microwell plates. Samples from patients treated for hematologic malignancies were studied. The chelation method was based on mobilizatio...

The cytotoxic effects of bleomycin on HeLa cells in culture were enhanced by incubation of the cells with benzamide, a potent inhibitor of poly(ADP-ribose) polymerase, at concentrations at which benzamide alone did not show any cytotoxicity. Benzamide plus bleomycin display enhanced therapeutic effects against Ehrlich ascites tumor cells in vivo. On daily treatment with various doses of bleomyc...

Pharmacokinetics In mice, high concentrations of bleomycin are found in the skin, lungs, kidneys, peritoneum and lymphatics. Tumour cells of the skin and lungs have been found to have high concentrations of bleomycin in contrast to the low concentrations found in haematopoietic tissue. The low concentrations of bleomycin found in bone marrow may be related to high levels of bleomycin degradativ...

Background: Varicose veins and the complications of venous disease are common disorders in humans. Objective: To study the effects of bleomycin as a potential new sclerosing agent and its adverse events in treating varicose veins. Methods: Bleomycin-loaded liposomes 0.1ml was injected in the dorsal ear veins of white New Zealand rabbits. Sodium tetradecyl sulfate was used as a positive cont...

Magnolol, a compound extracted from the Chinese medicinal herb Magnolia officinalis, has been proved to exert multiple pharmacological effects, including anti-oxidant and anti-inflammation activities. In this study, how it influenced bleomycin-induced lung fibrosis of rats was investigated. A single intratracheal instillation of bleomycin (5 mg/Kg, sacrificed 7 and 28 days post bleomycin instil...

BACKGROUND Oxidative stress plays an important role in the pathogenesis of bleomycin-induced lung fibrosis and many antioxidant agents have been used for the treatment of this disease in animals. OBJECTIVES To evaluate the antioxidant effects of pomegranate seed extract (PSE) on bleomycin treated rats. MATERIALS AND METHODS Male Spraque - Dawley rats were divided into 5 groups: rats in grou...

The role of cigarette smoking in the development and outcome of pulmonary fibrosis is uncertain. In this study, we aimed to assess the effect of route of bleomycin exposure in the development of pulmonary fibrosis and the effects of cigarette smoke on bleomycin-induced lung fibrosis. We studied five groups of rats; 1-control group, 2-intratracheal bleomycin (IT), 3intratracheal bleomycin plus c...

There are several lines of evidence indicating that the DNA-cleaving activity of bleomycin determines its cytotoxicity. (1) Most bleomycin analogs and derivatives which degrade isolated DNA are also active against living cells and vice versal) ; (2) some bacterial mutants selected for sensitivity to bleomycin lacked DNA-repair systems',') ; and (3) more degradation of DNA was observed in cells ...