Microsatellite markers were isolated using dual-suppression-PCR for the endangered species Excentrodendron hsienmu (Tiliaceae) to evaluate the genetic diversity and population structure of this species. A total of 11 polymorphic microsatellite loci were characterized in E. hsienmu. The number of alleles per locus ranged from 2 to 9, with an average of 5.27. The expected heterozygosity value ran...

We investigated the transferability of 20 Fragaria vesca microsatellite primer pairs to 13 Fragaria vesca accessions, six Fragaria species and ten commercially important species in Rosaceae. Genetic diversity studies were carried among 16 diploid Fragaria accessions using these polymorphic microsatellites. The average number of alleles amplified for a polymorphic locus was 4.7 with maximum bein...

Estimation of population heterozygosity and library construction induced mutation rate from expressed sequence tag collections. Abstract: Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population het...

Background: Moose ( Alces alces) colonized the North American continent from Asia less than 15,000 years ago, and spread across the boreal forest regions of Canada and the northern United States (US).  Contemporary populations have low genetic diversity, due either to low number of individuals in the original migration (founder effect), and/or subsequent population bottlenecks in North America....

To evaluate the genetic diversity of the Xinjiang Tarim red deer (Cervus elaphus yarkandensis) population, we analyzed the frequencies of microsatellite alleles. Samples were collected from 3 isolated populations in Xaya, Lopnur and Qarqan of Xinjiang. Although 10 microsatellite loci were examined, alleles of 133 to 190 base-pairs were detected for only 3 loci: BM5004, BM4208 and BM888. The ave...

Surveying the literature, the frequency distribution of single-locus heterozygosity among protein loci was examined in 95 vertebrate and 34 invertebrate species with the aim of testing the validity of the mutation-drift hypothesis. This distribution did not differ significantly from that expected under the mutation-drift hypothesis for any of the species examined when tested by the Kolmogorov-S...

Information on the genetic structure of marine species is essential for stock improvement programs. In orderto analyses the genetic diversity of the Hawksbill turtle (Eretmochelys imbricte) by the microsatellite geneticmethod, 64 samples were caught from the beaches located in Kish and Qeshm islands. Polymerase chainreactions (PCR) of genomic DNA extracted from the samples wer...

to clarify the genetic relationships among the lidia breed lineages and two main portuguese lidia bovine populations, casta portuguesa and brava dos açores, 24 autosomal microsatellites were analyzed in 120 samples. brava dos açores showed the highest observed and expected heterozygosity (0.73 and 0.70, respectively) while casta portuguesa showed the lowest observed and expected heterozygosity ...