in this research 130 sheep-dung samples were collected from kermani breed in area of kerman randomly. after processing and culture of samples, the produced colonies were studied morphologically and gram stain test and the genus of these bacteria were identified using biochemical tests. the clostridia strains isolates were included 30 percent of total samples i.e. clostridiums perfringense, bara...

roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...

Biochemical polymorphism study is a method of determination of genetic variation. This variability could be a basis for selection and subsequent genetic improvement in farm animals. The polymorphism in the intron 1 of chicken growth hormone (cGH) gene was investigated in the Iranian native fowls by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The...

The Hitachi SV1100 utilizes capillary electrophoresis on a microchip that is capable of rapidly sizing DNA fragments. Reproducibility of electrophoresis in different channels was shown by comparing the migration times of the internal controls, DNA fragments of 100 and 800 bp. The range of DNA sizing for this microchip is between 100 and 800 bp, and accuracy in sizing of a 322 bp DNA fragment of...

abstract background: fascioliasis, caused by fasciola hepatica and f. gigantica , has medical and economic importance in the world. molecular approaches comparing tradi­tional methods using for identification and characterization of fasciola spp. are precise and reliable. the aims of current study were molecular characterization of fasciola spp. in west azerbaijan province, iran and then compar...

Four different DNA loci were assessed for the detection of H. pylori by PCR on gastric biopsy specimens. PCR, with a primer specific 860 bp DNA fragment, was the most sensitive, with a detection limit of 0.02 pg H. pylori DNA, corresponding to approximately 10 organisms. Nested-PCR of the 860-bp DNA fragment was 10-fold more sensitive than single-step PCR. The sensitivity and specificity of the...

background and aims: tomato yellow leaf curl virus (tylcv) is one of the most destructive viruses of tomato that leads to reduced tomato yield up to 100% in tropical and subtropical regions. in this study, the complete sequence of tylcv isolate from hormozgan province, iran and its recombination evsent was determined. methods: tylcv infected tomato was collected from hormozgan province. total d...

DNA fragments Containing monkey DNA sequences have been isolated from defective SV40 genomes that carry host sequences in place of portions of the SV40 genome. The fragments were isolated by restriction endonuclease cleavage and contain segments homologous to sequences in both the highly repetitive and unique (or less repetitive) classes of monkey DNA. The complete nucleotide sequence of one su...

background: we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differen­tiate the pathogenic species, entamoeba histolytica , from the non-pathogenic species, e. dispar . methods: we developed a single pcr method for this purpose.  after investigation of genbank, primer pairs were de­signed from highly conserved regions of cysteine proteinase...

all species in the genus lysiphlebus förster (hymenoptera: braconidae, aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. a pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, l. fabarum marshall, collected from guilan province (iran)....