Aims: To measure the content of immunoreactive human pancreatic secretory trypsin inhibitor (irPSTI) in colonic carcinoma and adjacent normal colonic mucosa. Methods: From a stable hybridoma cell line producing monoclonal antibodies specific for human PSTI, a specific enzyme linked immunosorbent assay (ELISA) for human PSTI was developed. In a precipitation assay system these antibodies bound h...

Aims: To measure the content of immunoreactive human pancreatic secretory trypsin inhibitor (irPSTI) in colonic carcinoma and adjacent normal colonic mucosa. Methods: From a stable hybridoma cell line producing monoclonal antibodies specific for human PSTI, a specific enzyme linked immunosorbent assay (ELISA) for human PSTI was developed. In a precipitation assay system these antibodies bound h...

Polymorphisms in genes encoding P450 cytochrome enzymes may increase the risk of sporadic colorectal cancer (SCRC). Here we investigated the association between SCRC and CYP2E1 (PstI) and CYP1A1 (MspI) polymorphisms in a case-control study. Moreover, we sought to determine any possible associations between this disease and the sociodemographic factors. We included 273 individuals (74 patients a...

This paper presents a new method for estimating parameters of 3D lines from Plane Spatio-Temporal Image(PST1). A PSTI is created by using a slit to scan a 3D ST1 which is obtained by a camera mounted on a moving car. We have discussed the creation of PSTI with the cameramotion of straightline translation by using a hyperbolical slit and the 3D information acquisition before. In this paper, we g...

Mitochondrial DNA (mtDNA) deletions are a common cause of mitochondrial disorders and have been found to accumulate during normal aging. Despite the fact that hundreds of deletions have been characterized at the molecular level, their mechanisms of genesis are unknown. We tested the effect of double-strand breaks of muscle mtDNA by developing a mouse model in which a mitochondrially targeted re...

Pancreatic secretory trypsin inhibitor is a potent protease inhibitor which was originally identified in the pancreas. It has subsequently been shown to be present in mucus-secreting cells throughout the gastrointestinal tract and also in the kidney, lung and breast. Its major roles are likely to be to prevent premature activation of pancreatic proteases and to decrease the rate of mucus digest...

SOURCE/DESCRIPTION: pEl was subcloned from the cosmid pcosEMBLaAT(ref .1) and contains a 9.6kb-fragment of the cosmid extending from the EcoRI-site in in-tron A to an EcoRI-site 2.5kb 3' of the «-antitrypsin gene.Vector is pBR322. POLYMORPHISM: PstI identifies a 2-allele system with alternative bands at 5.0kb (PI) and at 3.2kb (P2) for a site within the a.-antitrypsin-like gene. Several constan...

The simplified AFLP method was developed and evaluated for identification and genetic diversity studies of wheat cultivars. Selective primers exploited in AFLP assay based on a single cutting enzyme PstI ((PstI)AFLP) generated total of 111 robust fragments, including 67 (60%) monomorphic and 12 (11%) cultivar-specific markers. Average similarity between 15 cultivars was 0.650, and varied from 0...

Figure S1. Additional validations of microarray predicted ESRP regulated splicing events. Semiquantitative RT-PCR was used to assay a set of more complex candidate alternative splicing events. Identities of PCR products are labeled to the right of the gels. The percent inclusion of the relevant exon(s), shaded in red, is given below each lane. For the mutually exclusive splicing events in the F...