Background Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we engineered Pichia pastoris, an industrial workhorse in heterologous enzyme p...

Poly-(R)-3-hydroxybutyric acid (PHB) was synthesized anaerobically in recombinant Escherichia coli. The host anaerobically accumulated PHB to more than 50% of its cell dry weight during cultivation in either growth or nongrowth medium. The maximum specific PHB production rate during growth-associated synthesis was approximately 2.3 +/- 0.2 mmol of PHB/g of residual cell dry weight/h. The by-pro...

The metabolism of glucose was examined in several clinical isolates of Neisseria gonorrhoeae. Radiorespirometric studies revealed that growing cells metabolized glucose by a combination on the Entner-Doudoroff and pentose phosphate pathways. A portion of the glyceraldehyde-3-phosphate formed via the Entner-Doudoroff pathway was recycled by conversion to glucose-6-phosphate. Subsequent catabolis...

• Tyrosol acetate and hydroxytyrosol have wide applications both as functional food components nutraceuticals. Construction of tyrosol biosynthetic pathway in E. coli . derived from virgin olive oil olives extract, However, they low bioavailability due to their absorption high metabolism human liver small intestine. Acetylation can effectively improve thus increase potential use the cosmeceutic...

Methane (CH4) has attracted attention as not only one of the hydrogen carriers in terms energy density, but also synthetic natural gas. In nature, decomposition organic compounds is performed with bacterial ecosystems that can produce CH4. Clostridium cellulovorans a decomposer was cultivated pig manure (PM) an unused biomass this study. As result high-performance liquid chromatography (HPLC) a...

Soluble manganese in the intermediate +III oxidation state (Mn3+ ) is a newly identified oxidant in anoxic environments, whereas acetate is a naturally abundant substrate that fuels microbial activity. Microbial populations coupling anaerobic acetate oxidation to Mn3+ reduction, however, have yet to be identified. We isolated a Shewanella strain capable of oxidizing acetate anaerobically with M...

Eubacterium acidaminophilum combines the oxidation of amino acids such as alanine or valine with the reduction of glycine to acetate in a two-substrate fermentation (Stickland reaction). In the absence of glycine, dense cell suspensions oxidized alanine or valine only to a small extent, with limited production of hydrogen and acetate. Experiments with 14C-labeled carbonate revealed that acetate...

Feeding experim ents using l3C-labelled sodium acetate precursors in CuCl2-treated broad bean (Vicia faba) cotyledons have demonstrated that the furanoacetylene phytoalexin wyerone is biosynthetically derived from seven intact acetate units. A further experiment using sodium [2H 3]acetate indicated the head of the chain, and showed the chain is analogous to that of a fatty acid precursor, any c...

In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK-PTA) of the major acetate synthesis pathways reduced acetate accumulation. The ackA-pta mutant strain also exhibits an increased lactate synthesis rate. Metabolic flux analysis suggested that the majority of excessive carbon flux was redirected through the lactate formation pat...