Hemicellulose hydrolysates, sugar-rich feedstocks used in biobutanol refinery, are normally obtained by adding sodium hydroxide in the hydrolyze process. However, the resulting high sodium concentration in the hydrolysate inhibits ABE (acetone-butanol-ethanol) fermentation, and thus limits the use of these low-cost feedstocks. We have thus studied the effect of high sodium on the metabolic beha...

The feasibility of substituting glucose with fructose as a carbon source in Escherichia coli fermentations was investigated. Glucose, the most commonly used sugar in bacterial cultivations, is well-known to pose a number of drawbacks; the most important of which is the Crabtree effect, which results in acidogenesis. Fructose, a glucose structural isomer, offers a reasonable alternative for gluc...

Abstract In this work, the effect of bioaugmentation on hydrolysis and acidogenesis efficiency bedding straw mixed with maize silage is examined. A plug-flow bioreactor was operated for 70 weeks as a reference feedstock subsequently an increasing content 30% 66% (w/w). Bioaugmentation two Paenibacillus species conducted at each process condition to investigate impact recalcitrant lignocellulosi...

Utilising ‘wastes’ as ‘resources’ is key to a circular economy. While there are multiple routes waste valorisation, anaerobic digestion (AD)—a biochemical means breakdown organic wastes in the absence of oxygen—is favoured due its capacity handle variety feedstocks. Traditional AD focuses on production biogas and fertiliser products; however, such low-value products combined with longer residen...

An anaerobic landfill leachate bioreactor was operated with crystalline cellulose and sterile landfill leachate until a steady state was reached. Cellulose hydrolysis, acidogenesis, and methanogenesis were measured. Microorganisms attached to the cellulose surfaces were hypothesized to be the cellulose hydrolyzers. 16S rRNA gene clone libraries were prepared from this attached fraction and also...

OBJECTIVES To evaluate the synergistic effect of Quercitrin and Deoxynojirimycin (DNJ) together with their individual inhibitory effect against virulence pathways of Streptococcus mutans. METHODOLOGY MICs of both the compounds were determined by the microdilution method, followed by their in vitrosynergy using checkerboard and time kill assay. The nature of interaction was classified as syner...