Organs with different levels of stiffness support the musculoskeletal system. Light microscopy cannot evaluate organ stiffness, whereas scanning acoustic (SAM) discriminates based on speed-of-sound (SOS) because sound waves pass faster in stiffer tissues. This study aimed to SOS imaging for orthopedic diseases using formalin-fixed paraffin-embedded sections. SAM uses unstained light microscopic...

Variations in the acoustic impedance throughout cells and tissue can be used to gain insight into cellular microstructures and the physiologic state of the cell. Ultrasound imaging can be used to create a map of the acoustic impedance, on which fluctuations can be used to help identify the dominant ultrasound scattering source in cells, providing information for ultrasound tissue characterizati...

An asymptotic description of the acoustic signature of a crack, breaking the surface of an otherwise homogeneous, isotropic elastic material, determined using either a line focus or a point focus scanning acoustic microscope is constructed. The incident focused beam is constructed as a Fourier integral that produces a specified profile in the focal plane. The wavefields scattered from the speci...

The development of acoustic subsurface atomic force microscopy, which promises three-dimensional imaging with single-digit nanometer resolution by the introduction ultrasound actuations to a conventional microscope, has come long way since its inception in early 1990s. Recent advances provide quantitative understanding different experimentally observed contrast mechanisms, paves for future appl...

We have constructed an atomic force microscope enabling one to image the topography of a sample, and to monitor simultaneously ultrasonic surface vibrations in the MHz range. For detection of the distribution of the ultrasonic vibration amplitude, a part of the position-sensing light beam reflected from the cantilever is directed to an external knife-edge detector. Acoustic images taken on the ...

We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advant...

This paper deals with metallographic studies and ultrasonic investigations of acoustic birefringence in the base metal heat affected zone welded specimens ASTM 1020 steel under uniaxial tension low-cycle fatigue. We examined microstructural changes on surface using optical microscope. It was found that development slip bands differs zone. The pulse echo method used for determination velocities ...