Traditionally, Mycobacterium tuberculosis or granulomas have to be evidenced in the pleural cavity in order to establish a diagnosis of tuberculous pleuritis. In recent years, adenosine deaminase has aided tremendously in the diagnosis of tuberculous pleural effusion, often obviating the need for microbiologic or histologic verification of the diagnosis. However, the fact that the sensitivity o...

An improved method was devised for the estimation of serum adenosine deaminase activity. The principle of the method consists in measurement of a liberated ammonia by the action of the deaminase with a direct colorimetric determination of ammonia concentration [Okuda et al.]. The technique is relatively easy to estimate the serum enzyme activity and can be applied for routine clinical use. The ...

Erythrocytes from 1 1 patients with presumptive diagnoses of transient erythroblastopenia of childhood were evaluated retrospectively (six) or prospectively (five) for a possible relationship between erythrocyte adenosine 5’monophosphate aminohydrolase. adenylic acid deaminase (AMP deaminase) activity and intracellular concentrations of adenine nucleotides. Older red blood cell (RBC) cohorts in...

A series of emissive ribonucleoside purine mimics, all comprised of an isothiazolo[4,3-d]pyrimidine core, was prepared using a divergent pathway involving a key Thorpe-Ziegler cyclization. In addition to an adenosine and a guanosine mimic, analogues of the noncanonical xanthosine, isoguanosine, and 2-aminoadenosine were also synthesized and found to be emissive. Isothiazolo 2-aminoadenosine, an...

The nature of the association of adenosine deaminase deficiency and severe combined immunodeficiency disease is reviewed . The basis for the molecular heterogeneity exhibited by adenosine deaminase in human tissue and the mechanisms whereby a deficiency of this activity results in the extreme perturbation of the immune system as observed in severe combined immunodeficiency are critically discus...

Inherited deficiencies of the enzymes adenosine deaminase (adenosine aminohydrolase; EC 3.5.4.4) and purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase; EC 2.4.2.1) preferentially interfere with lymphocyte development while sparing most other organ systems. Previous experiments have shown that through the action of specific kinases, nucleosides can be "trapped"...

Cultured leukemic T-lymphoblasts, incubated in the pres ence of inhibitors of adenosine deaminase, are exquisitely sensitive to growth inhibition by deoxyadenosine. An analogy between this phenomenon and human combined immunodefi ciency disease associated with inborn adenosine deaminase deficiency and the use of inhibitors of adenosine deaminase in the management of T-cell acute lymphoblastic l...

ceeds supply.@ Adenosine measurement is difficult in any given circumstance, as elements such as endothe hal cell and blood uptake and release, interstitial concentration, intracellular binding with SAH-hydro lase, and flow all influence venous adenosine concen trations. Adenosine is removed from tissues either by phos phorylation to AMP by adenosine kinase or by deam ination by adenosine deami...

OBJECTIVE Crimean-Congo hemorrhagic fever is an acute viral hemorrhagic fever with a high mortality rate. Despite increasing knowledge about hemorrhagic fever viruses, little is known about the pathogenesis of Crimean-Congo hemorrhagic fever. In this study, we measured serum adenosine deaminase and xanthine oxidase levels in Crimean-Congo hemorrhagic fever patients. METHODS Serum adenosine de...