Adenosine has been implicated to play a role in asthma in part through its ability to influence mediator release from mast cells. Most physiological roles of adenosine are mediated through adenosine receptors; however, the mechanisms by which adenosine influences mediator release from lung mast cells are not understood. We established primary murine lung mast cell cultures and used real-time RT...

Purinergic1 receptors were initially conceived to describe the likely existence of membrane receptors responding to adenosine triphosphate (ATP) (P2 receptors) or its breakdown product adenosine (P1 receptors) in causing nonadrenergic, noncholinergic relaxation of gut smooth muscle.1 Cloning of these receptors has yielded insights into their properties and signaling mechanisms, allowing classif...

Purinergic1 receptors were initially conceived to describe the likely existence of membrane receptors responding to adenosine triphosphate (ATP) (P2 receptors) or its breakdown product adenosine (P1 receptors) in causing nonadrenergic, noncholinergic relaxation of gut smooth muscle.1 Cloning of these receptors has yielded insights into their properties and signaling mechanisms, allowing classif...

Formyl peptide receptor-induced chemotaxis of neutrophils depends on the release of ATP and autocrine feedback through purinergic receptors. Here, we show that adrenergic receptor signaling requires similar purinergic feedback mechanisms. Real-time RT-PCR analysis revealed that human embryonic kidney (HEK)-293 cells express several subtypes of adrenergic (α(1)-, α(2)-, and β-receptors), adenosi...

Adenosine is known to exert dual actions on the afferent arteriole, eliciting vasoconstriction, by activating A1 receptors, and vasodilation at higher concentrations, by activating lower-affinity A2 receptors. We could demonstrate both of these known adenosine responses in the in vitro perfused hydronephrotic rat kidney. Thus, 1.0 μM adenosine elicited a transient vasoconstriction blocked by 8-...